• STJ70102 (0. 1µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70102 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70102 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-DDAH2 antibody (C-Term) (STJ70102)

SKU:
STJ70102-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-DDAH2 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:16000.
WB: Approx 28kDa band observed in Human Lung lysates (calculated MW of 29.6kDa according to NP_039268.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. Preliminar
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: DDAH2
Gene ID: 23564
Uniprot ID: DDAH2_HUMAN
Immunogen Region: C-Term
Accession Number: NP_039268.1
Immunogen Sequence: SSLCLVLSTRPHS
Post Translational Modifications Phosphorylated by TBK1. Phosphorylation inhibits the translocation into the mitochondrion upon Sendai viral infection.
Function Putative hydrolase with unknown substrate (Probable). Does not hydrolyze N(G),N(G)-dimethyl-L-arginine (ADMA) which acts as an inhibitor of NOS. In endothelial cells, induces expression of vascular endothelial growth factor (VEGF) via phosphorylation of the transcription factor SP1 by PKA in a process that is independent of NO and NO synthase. Similarly, enhances pancreatic insulin secretion through SP1-mediated transcriptional up-regulation of secretagogin/SCGN, an insulin vesicle docking protein. Upon viral infection, relocates to mitochondria where it promotes mitochondrial fission through activation of DNM1L leading to the inhibition of innate response activation mediated by MAVS.
Protein Name Putative Hydrolase Ddah2
Ddahii
Inactive N(G -N(G-Dimethylarginine Dimethylaminohydrolase 2
Ddah-2
Inactive Dimethylarginine Dimethylaminohydrolase 2
Protein G6a
S-Phase Protein
Cellular Localisation Cytoplasm
Mitochondrion
Translocates From Cytosol To Mitochondrion Upon Il1b Stimulation In Chondrocytes
(Microbial Infection) Translocates To The Mitochondrion Upon Sendai Viral Infection
Alternative Antibody Names Anti-Putative Hydrolase Ddah2 antibody
Anti-Ddahii antibody
Anti-Inactive N(G -N(G-Dimethylarginine Dimethylaminohydrolase 2 antibody
Anti-Ddah-2 antibody
Anti-Inactive Dimethylarginine Dimethylaminohydrolase 2 antibody
Anti-Protein G6a antibody
Anti-S-Phase Protein antibody
Anti-DDAH2 antibody
Anti-DDAH antibody
Anti-G6A antibody
Anti-NG30 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance