• STJ70102 (0. 1µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70102 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70102 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-DDAH2 antibody (C-Term) (STJ70102)

SKU:
STJ70102-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-DDAH2 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
IF-Strong expression of the protein seen in the cytoplasm of A431 cells. 10µg/ml
FC-Flow cytometric analysis of A431 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: DDAH2
Gene ID: 23564
Uniprot ID: DDAH2_HUMAN
Immunogen Region: C-Term
Accession Number: NP_039268.1
Immunogen Sequence: SSLCLVLSTRPHS
Function Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Protein Name N(G -N(G-Dimethylarginine Dimethylaminohydrolase 2
Ddah-2
Dimethylarginine Dimethylaminohydrolase 2
Ddahii
Dimethylargininase-2
Protein G6a
S-Phase Protein
Database Links Reactome: R-HSA-203615
Cellular Localisation Cytoplasm
Mitochondrion
Translocates From Cytosol To Mitochondrion Upon Il-1beta Stimulation In Chondrocytes
Alternative Antibody Names Anti-N(G -N(G-Dimethylarginine Dimethylaminohydrolase 2 antibody
Anti-Ddah-2 antibody
Anti-Dimethylarginine Dimethylaminohydrolase 2 antibody
Anti-Ddahii antibody
Anti-Dimethylargininase-2 antibody
Anti-Protein G6a antibody
Anti-S-Phase Protein antibody
Anti-DDAH2 antibody
Anti-DDAH antibody
Anti-G6A antibody
Anti-NG30 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance