• Western blot analysis of various lysates using DAZAP2 Rabbit polyclonal antibody (STJ116479) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DAZAP2 antibody (1-168) (STJ116479)

SKU:
STJ116479

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DAZAP2 (1-168) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DAZAP2
Gene ID: 9802
Uniprot ID: DAZP2_HUMAN
Immunogen Region: 1-168
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human DAZAP2 (NP_055579.1).
Immunogen Sequence: MNSKGQYPTQPTYPVQPPGN PVYPQTLHLPQAPPYTDAPP AYSELYRPSFVHPGAATVPT MSAAFPGASLYLPMAQSVAV GPLGSTIPMAYYPVGPIYPP GSTVLVEGGYDAGARFGAGA TAGNIPPPPPGCPPNAAQLA VMQGANVLVTQRKGNFFMGG SDGGYTIW
Tissue Specificity Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Down-regulated in multiple myeloma.
Post Translational Modifications Ubiquitinated. Ubiquitinated by SMURF2, leading to proteasomal degradation. Ubiquitinated by NEDD4, leading to proteasomal degradation. Following DNA damage, phosphorylated by HIPK2 which promotes DAZAP2 localization to the nucleus, reduces interaction of DAZAP2 with HIPK2 and SIAH1, and prevents DAZAP2-dependent ubiquitination of HIPK2 by E3 ubiquitin-protein ligase SIAH1 and subsequent HIPK2 proteasomal degradation.
Function In unstressed cells, promotes SIAH1-mediated polyubiquitination and degradation of the serine/threonine-protein kinase HIPK2, probably by acting as a loading factor that potentiates complex formation between HIPK2 and ubiquitin ligase SIAH1. In response to DNA damage, localizes to the nucleus following phosphorylation by HIPK2 and modulates the expression of a subset of TP53/p53 target genes by binding to TP53 at target gene promoters. This limits the expression of a number of cell death-mediating TP53 target genes, reducing DNA damage-induced cell death. Enhances the binding of transcription factor TCF7L2/TCF4, a Wnt signaling pathway effector, to the promoters of target genes. Plays a role in stress granule formation.
Protein Name Daz-Associated Protein 2
Deleted In Azoospermia-Associated Protein 2
Proline-Rich Transcript In Brain Protein
Cellular Localisation Cytoplasm
Nucleus
Nucleus Speckle
Nuclear Body
Stress Granule
Predominantly Nuclear In Macrophages
Stimulation Of Il17rb With Its Ligand Il17e Induces Accumulation In The Cytoplasm
Predominantly Cytoplasmic When Unphosphorylated And Localizes To The Nucleus Following Phosphorylation By Hipk2
Localizes To Stress Granules Under Cellular Stress Conditions
Alternative Antibody Names Anti-Daz-Associated Protein 2 antibody
Anti-Deleted In Azoospermia-Associated Protein 2 antibody
Anti-Proline-Rich Transcript In Brain Protein antibody
Anti-DAZAP2 antibody
Anti-KIAA0058 antibody
Anti-PRTB antibody

Information sourced from Uniprot.org

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