Western blot analysis of 293F, using DACH1 antibody (STJ28975) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DACH1 antibody (450-570) (STJ28975)

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STJ28975
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DACH1
Gene ID: 1602
Uniprot ID: DACH1_HUMAN
Immunogen Region: 450-570
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 450-570 of human DACH1 (NP_542937.2).
Immunogen Sequence: EGRRPGSHPSSHRSSSVSSS PARTESSSDRIPVHQNGLSM NQMLMGLSPNVLPGPKEGDL AGHDMGHESKRMHIEKDETP LSTPTARDSLDKLSLTGHGQ PLPPGFPSPFLFPDGLSSIE T
Tissue Specificity Widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen.
Function Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain.
Protein Name Dachshund Homolog 1
Dach1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dachshund Homolog 1 antibody
Anti-Dach1 antibody
Anti-DACH1 antibody
Anti-DACH antibody

Information sourced from Uniprot.org

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