• Confocal imaging of paraffin-embedded Human tonsil tissue using Cytokeratin 2e (KRT2) Rabbit monoclonal antibody (STJ11103497, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of paraffin-embedded mouse skin using Cytokeratin 2e (KRT2) Rabbit monoclonal antibody (STJ11103497) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded human skin using Cytokeratin 2e (KRT2) Rabbit monoclonal antibody (STJ11103497) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat skin using Cytokeratin 2e (KRT2) Rabbit monoclonal antibody (STJ11103497) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Cytokeratin 2e (KRT2) in paraffin-embedded mouse skin using Cytokeratin 2e (KRT2) Rabbit monoclonal antibody (STJ11103497) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from A-431 cells, using Cytokeratin 2e (KRT2) Rabbit monoclonal antibody (STJ11103497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Cytokeratin 2e antibody (293-422) [S7MR] (STJ11103497)

SKU:
STJ11103497

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cytokeratin 2e (KRT2) (293-422) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT2
Gene ID: 3849
Uniprot ID: K22E_HUMAN
Immunogen Region: 293-422
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 293-422 of humanKRT2 (P35908).
Immunogen Sequence: YMIKVELQSKVDLLNQEIEF LKVLYDAEISQIHQSVTDTN VILSMDNSRNLDLDSIIAEV KAQYEEIAQRSKEEAEALYH SKYEELQVTVGRHGDSLKEI KIEISELNRVIQRLQGEIAH VKKQCKNVQD
Tissue Specificity Expressed in the upper spinous and granular suprabasal layers of normal adult epidermal tissues from most body sites including thigh, breast nipple, foot sole, penile shaft and axilla. Not present in foreskin, squamous metaplasias and carcinomas. Expression in hypertrophic and keloid scars begins in the deepest suprabasal layer. Weakly expressed in normal gingiva and tongue, however expression is induced in benign keratoses of lingual mucosa and in mild-to-moderate oral dysplasia with orthokeratinization.
Function Probably contributes to terminal cornification. Associated with keratinocyte activation, proliferation and keratinization. Required for maintenance of corneocytes and keratin filaments in suprabasal keratinocytes in the epidermis of the ear, potentially via moderation of expression and localization of keratins and their partner proteins. Plays a role in the establishment of the epidermal barrier on plantar skin.
Protein Name Keratin - Type Ii Cytoskeletal 2 Epidermal
Cytokeratin-2e
Ck-2e
Epithelial Keratin-2e
Keratin-2 Epidermis
Keratin-2e
K2e
Type-Ii Keratin Kb2
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 2 Epidermal antibody
Anti-Cytokeratin-2e antibody
Anti-Ck-2e antibody
Anti-Epithelial Keratin-2e antibody
Anti-Keratin-2 Epidermis antibody
Anti-Keratin-2e antibody
Anti-K2e antibody
Anti-Type-Ii Keratin Kb2 antibody
Anti-KRT2 antibody
Anti-KRT2A antibody
Anti-KRT2E antibody

Information sourced from Uniprot.org

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