• Confocal imaging of paraffin-embedded Rat eyeball tissue using Cytokeratin 12 (KRT12) Rabbit monoclonal antibody (STJ11103015, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Mouse eyeball tissue using Cytokeratin 12 (KRT12) Rabbit monoclonal antibody (STJ11103015, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of Cytokeratin 12 (KRT12) in paraffin-embedded Mouse cornea using Cytokeratin 12 (KRT12) (KRT12) Rabbit monoclonal antibody (STJ11103015) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Cytokeratin 12 (KRT12) in paraffin-embedded rat cornea using Cytokeratin 12 (KRT12) (KRT12) Rabbit monoclonal antibody (STJ11103015) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat eye, using Cytokeratin 12 (KRT12) Rabbit monoclonal antibody (STJ11103015) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Cytokeratin 12 antibody (250-494) [S5MR] (STJ11103015)

SKU:
STJ11103015

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cytokeratin 12 (KRT12) (250-494) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT12
Gene ID: 3859
Uniprot ID: K1C12_HUMAN
Immunogen Region: 250-494
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-494 of human Cytokeratin 12 (KRT12) (NP_000214.1).
Immunogen Sequence: EMQIESLNEELAYMKKNHED ELQSFRVGGPGEVSVEMDAA PGVDLTRLLNDMRAQYETIA EQNRKDAEAWFIEKSGELRK EISTNTEQLQSSKSEVTDLR RAFQNLEIELQSQLAMKKSL EDSLAEAEGDYCAQLSQVQQ LISNLEAQLLQVRADAERQN VDHQRLLNVKARLELEIETY RRLLDGEAQGDGLEESLFVT DSKSQAQSTDSSKDPTKTRK IKTVVQEMVNGEVVSSQVQ
Tissue Specificity Expressed in the corneal epithelium (at protein level).
Function Involved in corneal epithelium organization, integrity and corneal keratin expression.
Protein Name Keratin - Type I Cytoskeletal 12
Cytokeratin-12
Ck-12
Keratin-12
K12
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 12 antibody
Anti-Cytokeratin-12 antibody
Anti-Ck-12 antibody
Anti-Keratin-12 antibody
Anti-K12 antibody
Anti-KRT12 antibody

Information sourced from Uniprot.org

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