| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IF/ICC:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: |
CYP8B1 |
| Gene ID: |
1582 |
| Uniprot ID: |
CP8B1_HUMAN |
| Immunogen Region: |
311-410 aa |
| Specificity: |
A synthetic peptide corresponding to a sequence within amino acids 311-410 of human CYP8B1 (NP_004382.2). |
| Immunogen Sequence: |
ATQVLGEARLETKQSFAFKL GALQHTPVLDSVVEETLRLR AAPTLLRLVHEDYTLKMSSG QEYLFRHGDILALFPYLSVH MDPDIHPEPTVFKYDRFLN |
| Tissue Specificity | Liver. |
| Function | A cytochrome P450 monooxygenase involved in primary bile acid biosynthesis. Catalyzes the 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one, an intermediate metabolite in cholic acid biosynthesis. Controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH--hemoprotein reductase). |
| Protein Name | 7-Alpha-Hydroxycholest-4-En-3-One 12-Alpha-Hydroxylase7-Alpha-Hydroxy-4-Cholesten-3-One 12-Alpha-HydroxylaseCypviiib1Cytochrome P450 8b1Sterol 12-Alpha-Hydroxylase |
| Database Links | Reactome: R-HSA-193368Reactome: R-HSA-193775Reactome: R-HSA-193807Reactome: R-HSA-197264Reactome: R-HSA-211979Reactome: R-HSA-211994Reactome: R-HSA-2162123 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinMicrosome Membrane |
| Alternative Antibody Names | Anti-7-Alpha-Hydroxycholest-4-En-3-One 12-Alpha-Hydroxylase antibodyAnti-7-Alpha-Hydroxy-4-Cholesten-3-One 12-Alpha-Hydroxylase antibodyAnti-Cypviiib1 antibodyAnti-Cytochrome P450 8b1 antibodyAnti-Sterol 12-Alpha-Hydroxylase antibodyAnti-CYP8B1 antibodyAnti-CYP12 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
