Anti-CYP4F11 antibody (60-130) (STJ11104057)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Cytochrome P450 4f11 (60-130) antibody for use in IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CYP4F11 |
| Gene ID | 57834 |
| Uniprot ID | CP4FB_HUMAN |
| Immunogen Region | 60-130 |
| Immunogen Sequence | FWGHQGLVTPTEEGMKTLTQ LVTTYPQGFKLWLGPTFPLL ILCHPDIIRPITSASAAVAP KDMIFYGFLKP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 60-130 of human CYP4F11 (NP_001122404.1). |
Additional Info
| Tissue Specificity | Expressed mainly in human liver, followed by kidney, heart, and skeletal muscle. |
| Post Translational Modifications | 4-hydroxynonenal conjugation impairs substrate binding and the long-chain fatty acid omega-monooxygenase activity. |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids and their oxygenated derivatives (oxylipins). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). Catalyzes with high efficiency the oxidation of the terminal carbon (omega-oxidation) of 3-hydroxy fatty acids, such as 3-hydroxyhexadecanoic and 3-hydroxyoctadecanoic acids, likely participating in the biosynthesis of long-chain 3-hydroxydicarboxylic acids. Omega-hydroxylates and inactivates phylloquinone (vitamin K1), and menaquinone-4 (MK-4, a form of vitamin K2), both acting as cofactors in blood coagulation. Metabolizes with low efficiciency fatty acids, including (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) and its oxygenated metabolite 8-hydroxyeicosatetraenoic acid (8-HETE). Catalyzes N- and O-demethylation of drugs such as erythromycin, benzphetamine, ethylmorphine, chlorpromazine, imipramine and verapamil. Catalyzes the oxidation of dialkylresorcinol 2. |
| Protein Name | Cytochrome P450 4f11Cypivf113-Hydroxy Fatty Acids Omega-Hydroxylase Cyp4f11Docosahexaenoic Acid Omega-HydroxylaseLong-Chain Fatty Acid Omega-MonooxygenasePhylloquinone Omega-Hydroxylase Cyp4f11 |
| Database Links | Reactome: R-HSA-211935Reactome: R-HSA-211958Reactome: R-HSA-211979Reactome: R-HSA-2142691 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinMicrosome Membrane |
| Alternative Antibody Names | Anti-Cytochrome P450 4f11 antibodyAnti-Cypivf11 antibodyAnti-3-Hydroxy Fatty Acids Omega-Hydroxylase Cyp4f11 antibodyAnti-Docosahexaenoic Acid Omega-Hydroxylase antibodyAnti-Long-Chain Fatty Acid Omega-Monooxygenase antibodyAnti-Phylloquinone Omega-Hydroxylase Cyp4f11 antibodyAnti-CYP4F11 antibody |
Information sourced from Uniprot.org