Anti-CYP2E1 antibody (234-493) (STJ23323)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CYP2E1 (234-493) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CYP2E1 |
| Gene ID | 1571 |
| Uniprot ID | CP2E1_HUMAN |
| Immunogen Region | 234-493 |
| Immunogen Sequence | KVIKNVAEVKEYVSERVKEH HQSLDPNCPRDLTDCLLVEM EKEKHSAERLYTMDGITVTV ADLFFAGTETTSTTLRYGLL ILMKYPEIEEKLHEEIDRVI GPSRIPAIKDRQEMPYMDAV VHEIQRFITLVPSNLPHEAT RDTIFRGYLIPKGTVVVPTL DSVLYDNQEFPDPEKFKPEH FLNENGKFKYSDYFKPFSTG KRVCAGEGLARMELFLLLCA ILQHFNLKPLVDPKDIDLS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 234-493 of human CYP2E1 (NP_000764.1). |
Additional Info
| Function | A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics (Probable). |
| Protein Name | Cytochrome P450 2e14-Nitrophenol 2-HydroxylaseCypiie1Cytochrome P450-J |
| Database Links | Reactome: R-HSA-211981Reactome: R-HSA-211999Reactome: R-HSA-9027307Reactome: R-HSA-9749641Reactome: R-HSA-9753281 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinMicrosome MembraneMitochondrion Inner MembranePost-Translationally Targeted To MitochondriaTomm70 Is Required For The Translocation Across The Mitochondrial Outer MembraneAfter Translocation Into The MatrixAssociates With The Inner Membrane As A Membrane Extrinsic Protein |
| Alternative Antibody Names | Anti-Cytochrome P450 2e1 antibodyAnti-4-Nitrophenol 2-Hydroxylase antibodyAnti-Cypiie1 antibodyAnti-Cytochrome P450-J antibodyAnti-CYP2E1 antibodyAnti-CYP2E antibody |
Information sourced from Uniprot.org