Anti-CYP27A1 antibody (431-530) [S5065RM] (STJ11105065)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Sterol 26-Hydroxylase-Mitochondrial (431-530) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5065RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CYP27A1 |
| Gene ID | 1593 |
| Uniprot ID | CP27A_HUMAN |
| Immunogen Region | 431-530 |
| Immunogen Sequence | VSRDPTAFSEPESFQPHRWL RNSQPATPRIQHPFGSVPFG YGVRACLGRRIAELEMQLLL ARLIQKYKVVLAPETGELKS VARIVLVPNKKVGLQFLQRQ |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 431-530 of human CYP27A1 (NP_000775.1). |
Additional Info
| Tissue Specificity | Expressed in the neural retina and underlying retinal pigment epithelium (at protein level). Expressed in the gray and white matter of cerebellum (at protein level). |
| Function | Cytochrome P450 monooxygenase that catalyzes regio- and stereospecific hydroxylation of cholesterol and its derivatives. Hydroxylates (with R stereochemistry) the terminal methyl group of cholesterol side-chain in a three step reaction to yield at first a C26 alcohol, then a C26 aldehyde and finally a C26 acid. Regulates cholesterol homeostasis by catalyzing the conversion of excess cholesterol to bile acids via both the 'neutral' (classic) and the 'acid' (alternative) pathways. May also regulate cholesterol homeostasis via generation of active oxysterols, which act as ligands for NR1H2 and NR1H3 nuclear receptors, modulating the transcription of genes involved in lipid metabolism. Plays a role in cholestanol metabolism in the cerebellum. Similarly to cholesterol, hydroxylates cholestanol and may facilitate sterol diffusion through the blood-brain barrier to the systemic circulation for further degradation. Also hydroxylates retinal 7-ketocholesterol, a noxious oxysterol with pro-inflammatory and pro-apoptotic effects, and may play a role in its elimination from the retinal pigment epithelium. May play a redundant role in vitamin D biosynthesis. Catalyzes 25-hydroxylation of vitamin D3 that is required for its conversion to a functionally active form. |
| Protein Name | Sterol 26-Hydroxylase - Mitochondrial5-Beta-Cholestane-3-Alpha -7-Alpha -12-Alpha-Triol 26-HydroxylaseCytochrome P-450c27/25Cytochrome P450 27Sterol 27-HydroxylaseVitamin D(3 25-Hydroxylase |
| Database Links | Reactome: R-HSA-193368Reactome: R-HSA-193775Reactome: R-HSA-193807Reactome: R-HSA-211976Reactome: R-HSA-5578996 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinPost-Translationally Targeted To MitochondriaAll Three Of The Receptor Proteins In The Tom ComplexTomm70Tomm20 And Tomm22 Are Required For The Translocation Across The Mitochondrial Outer MembraneAfter Translocation Into The MatrixAssociates With The Inner Membrane As A Membrane Extrinsic Protein |
| Alternative Antibody Names | Anti-Sterol 26-Hydroxylase - Mitochondrial antibodyAnti-5-Beta-Cholestane-3-Alpha -7-Alpha -12-Alpha-Triol 26-Hydroxylase antibodyAnti-Cytochrome P-450c27/25 antibodyAnti-Cytochrome P450 27 antibodyAnti-Sterol 27-Hydroxylase antibodyAnti-Vitamin D(3 25-Hydroxylase antibodyAnti-CYP27A1 antibodyAnti-CYP27 antibody |
Information sourced from Uniprot.org