Host: | Rabbit |
Applications: | WB/IF/ICC/IP/ELISA |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
Purification: | Affinity purification |
Concentration: | Lot specific |
Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | CCND1 |
Gene ID: | 595 |
Uniprot ID: | CCND1_HUMAN |
Immunogen Region: | 200-295 |
Specificity: | A synthetic peptide corresponding to a sequence within amino acids 200-295 of human Cyclin D1 (NP_444284.1). |
Immunogen Sequence: | PSMVAAGSVVAAVQGLNLRS PNNFLSYYRLTRFLSRVIKC DPDCLRACQEQIEALLESSL RQAQQNMDPKAAEEEEEEEE EVDLACTPTDVRDVDI |
Post Translational Modifications | Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation by the DCX(AMBRA1) complex. It also plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex following DNA damage. Ubiquitinated at Lys-269 by the DCX(AMBRA1) complex during the transition from G1 to S cell phase, leading to its degradation: ubiquitination is dependent on Thr-286 phosphorylation. The DCX(AMBRA1) complex represents the major regulator of CCND1 stability during the G1/S transition. Also ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB. Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. SCF-type ubiquitination is dependent on Thr-286 phosphorylation. Ubiquitinated also by UHRF2 apparently in a phosphorylation-independent manner. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2.leading to its stabilization. |
Function | Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner. |
Protein Name | G1/S-Specific Cyclin-D1 B-Cell Lymphoma 1 Protein Bcl-1 Bcl-1 Oncogene Prad1 Oncogene |
Database Links | Reactome: R-HSA-187577 Reactome: R-HSA-1912408 Reactome: R-HSA-3214858 Reactome: R-HSA-6785807 Reactome: R-HSA-69231 Reactome: R-HSA-75815 Reactome: R-HSA-8849470 Reactome: R-HSA-8853884 Reactome: R-HSA-8878166 Reactome: R-HSA-8934593 Reactome: R-HSA-8951430 Reactome: R-HSA-8951936 Reactome: R-HSA-9018519 Reactome: R-HSA-9634638 Reactome: R-HSA-9661069 Reactome: R-HSA-9754119 Reactome: R-HSA-9825892 |
Cellular Localisation | Nucleus Cytoplasm Nucleus Membrane Cyclin D-Cdk4 Complexes Accumulate At The Nuclear Membrane And Are Then Translocated To The Nucleus Through Interaction With Kip/Cip Family Members |
Alternative Antibody Names | Anti-G1/S-Specific Cyclin-D1 antibody Anti-B-Cell Lymphoma 1 Protein antibody Anti-Bcl-1 antibody Anti-Bcl-1 Oncogene antibody Anti-Prad1 Oncogene antibody Anti-CCND1 antibody Anti-BCL1 antibody Anti-PRAD1 antibody |
Information sourced from Uniprot.org