| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Cyclin D-type binding-protein 1 (322-333) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 40kDa band observed in Human Placenta lysates (calculated MW of 40.3kDa according to NP_036274.3). Recommended concentration: 0.3-1µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CCNDBP1 |
| Gene ID: | 23582 |
| Uniprot ID: | CCDB1_HUMAN |
| Immunogen Region: | 322-333 |
| Accession Number: | NP_036274.3 |
| Immunogen Sequence: | TKASHVTPQPED |
| Post Translational Modifications | Phosphorylated. |
| Function | May negatively regulate cell cycle progression. May act at least in part via inhibition of the cyclin-D1/CDK4 complex, thereby preventing phosphorylation of RB1 and blocking E2F-dependent transcription. |
| Protein Name | Cyclin-D1-Binding Protein 1Grap2 And Cyclin-D-Interacting ProteinHuman Homolog Of Maid |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Cyclin-D1-Binding Protein 1 antibodyAnti-Grap2 And Cyclin-D-Interacting Protein antibodyAnti-Human Homolog Of Maid antibodyAnti-CCNDBP1 antibodyAnti-DIP1 antibodyAnti-GCIP antibodyAnti-HHM antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and ETFA knockout (KO) 293T cells, using [KO Validated] ETFA Rabbit polyclonal antibody (STJ11100813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89749/358226/STJ11100813_1__44625.1713122552.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ETFA knockout (KO) 293T cells, using [KO Validated] ETFA Rabbit polyclonal antibody (STJ11100813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
