| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal anti-Cytochrome b-245 light chain alpha chain (80-160 aa) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CYBA |
| Gene ID: | 1535 |
| Uniprot ID: | CY24A_HUMAN |
| Immunogen Region: | 80-160 aa |
| Specificity: | CY24A Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from the human protein at the amino acid range 80-160 |
| Function | Subunit of NADPH oxidase complexes that is required for the NADPH oxidase activity that generates, in various cell types, superoxide from molecular oxygen utilizing NADPH as an electron donor. Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox. Aassociates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide. |
| Protein Name | Cytochrome B-245 Light ChainCytochrome B(558 Alpha ChainCytochrome B558 Subunit AlphaNeutrophil Cytochrome B 22 Kda PolypeptideSuperoxide-Generating Nadph Oxidase Light Chain SubunitP22 Phagocyte B-CytochromeP22-PhoxP22phox |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-1236973Reactome: R-HSA-3299685Reactome: R-HSA-4420097Reactome: R-HSA-5668599Reactome: R-HSA-6798695Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423Reactome: R-HSA-9673324 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Cytochrome B-245 Light Chain antibodyAnti-Cytochrome B(558 Alpha Chain antibodyAnti-Cytochrome B558 Subunit Alpha antibodyAnti-Neutrophil Cytochrome B 22 Kda Polypeptide antibodyAnti-Superoxide-Generating Nadph Oxidase Light Chain Subunit antibodyAnti-P22 Phagocyte B-Cytochrome antibodyAnti-P22-Phox antibodyAnti-P22phox antibodyAnti-CYBA antibody |
Information sourced from Uniprot.org
