| Host: | Goat |
| Applications: | WB/IHC-F/IHC-P/IF/IEM |
| Reactivity: | Human/Rat/Mouse/Monkey/Canine |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Cathepsin D (275aa C-Term) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immune Electron Microscopy research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: | 3 mg/mL |
| Dilution Range: | WB 1:250-1:1000IF 1:50-1:200IHC-P 1:200-1:1000IHC-F 1:200-1:1000IEM 1:50-1:200Romagnoli M Bresson L Di-Cicco A et al. Development 2020 Jan. PMID: 31988184Pelkonen L Sato K Reinisalo M et al. Mol Pharm 2017 Mar. PMID: 281 |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: | CTSD |
| Gene ID: | 1509 |
| Uniprot ID: | CATD_HUMAN |
| Immunogen Region: | 275aa C-Term |
| Accession Number: | ENSG00000117984 |
| Specificity: | This antibody gives a positive signal in the following human (Jurkat, HT1080, HUH, MDA-MB-231, ARPE19, SH-SY5Y) , canine (MDCK) and monkey (COS-7) whole cell lysates. |
| Immunogen: | Purified recombinant peptide derived from within residues 275 aa to the C-terminus of human Cathepsin D produced in E. coli. |
| Immunogen Sequence: | DQVEVASGLTLCKEGCEAIV DTGTSLMVGPVDEVRELQKA IGAVPLIQGEYMIPCEKVST LPAITLKLGGKGYKLSPEDY TLKVSQAGKTLCLSGFMGMD IPPPSGPLWILGDVFIGRYY TVFDRDNNRVGFAEAARL |
| Tissue Specificity | Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level). |
| Post Translational Modifications | N- and O-glycosylated. Undergoes proteolytic cleavage and activation by ADAM30. As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified. |
| Function | Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. |
| Protein Name | Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-2022377Reactome: R-HSA-2132295Reactome: R-HSA-6798695Reactome: R-HSA-77387Reactome: R-HSA-9018519 |
| Cellular Localisation | LysosomeMelanosomeSecretedExtracellular SpaceIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvIn Aortic SamplesDetected As An Extracellular Protein Loosely Bound To The Matrix |
| Alternative Antibody Names | Anti-Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain antibodyAnti-CTSD antibodyAnti-CPSD antibody |
Information sourced from Uniprot.org
