• Anti-CTSD antibody at 1:500 dilution; endogenous CTSD (100 µg per lane) and transfected 293FT cell lysate (at 30 µg per lane) ; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunoperoxidase of polyclonal antibody to CTSD (1:200) on paraformaldehyde-fixed paraffin-embedded mouse lung
  • Immunofluorescence – anti-CTSD antibody at 1:100 dilution in RAW264.7 cells; cells were fixed with PFA and permeabilized with 0.05% saponin
  • Immunofluorescence – anti-CTSD antibody at 1:100 dilution in NHI:3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin
  • Immunogold labeling using melanocytes and anti-CTSD antibody
  • Immunofluorescence – anti-CTSD antibody at 1/100 dilution in RAW264. 7 cells; cells were fixed with PFA and permeabilized with 0. 05% saponin;
  • Immunohistochemistry of mouse spleen using anti-CTSD antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTSD antibody at 1:500/DAB detection.
  • Immunohistochemistry of mouse brain using anti-CTSD antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTSD antibody at 1:500/DAB detection.
  • Immunohistochemistry of mouse lung using anti-CTSD antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTSD antibody at 1:500/DAB detection.
  • Immunohistochemistry of mouse stomach using anti-CTSD antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTSD antibody at 1:500/DAB detection.
  • Immunohistochemistry of mouse eye using anti-CTSD antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTSD antibody at 1:500/DAB detection.
  • Anti-CTSD antibody at 1/500 dilution; endogenous CTSD (50 µg per lane) ; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

Anti-CTSD antibody (275aa C-Term) (STJ140018)

SKU:
STJ140018

£86.50 - £534.50
Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF/IEM
Reactivity: Human/Rat/Mouse/Monkey/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Cathepsin D (275aa C-Term) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immune Electron Microscopy research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/mL
Dilution Range: WB 1:250-1:1000
IF 1:50-1:200
IHC-P 1:200-1:1000
IHC-F 1:200-1:1000
IEM 1:50-1:200
Romagnoli M Bresson L Di-Cicco A et al. Development 2020 Jan. PMID: 31988184
Pelkonen L Sato K Reinisalo M et al. Mol Pharm 2017 Mar. PMID: 281
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Gene Symbol: CTSD
Gene ID: 1509
Uniprot ID: CATD_HUMAN
Immunogen Region: 275aa C-Term
Accession Number: ENSG00000117984
Specificity: This antibody gives a positive signal in the following human (Jurkat, HT1080, HUH, MDA-MB-231, ARPE19, SH-SY5Y) , canine (MDCK) and monkey (COS-7) whole cell lysates.
Immunogen: Purified recombinant peptide derived from within residues 275 aa to the C-terminus of human Cathepsin D produced in E. coli.
Immunogen Sequence: DQVEVASGLTLCKEGCEAIV DTGTSLMVGPVDEVRELQKA IGAVPLIQGEYMIPCEKVST LPAITLKLGGKGYKLSPEDY TLKVSQAGKTLCLSGFMGMD IPPPSGPLWILGDVFIGRYY TVFDRDNNRVGFAEAARL
Tissue Specificity Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level).
Post Translational Modifications N- and O-glycosylated. Undergoes proteolytic cleavage and activation by ADAM30. As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified.
Function Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Protein Name Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-2022377
Reactome: R-HSA-2132295
Reactome: R-HSA-6798695
Reactome: R-HSA-77387
Reactome: R-HSA-9018519
Cellular Localisation Lysosome
Melanosome
Secreted
Extracellular Space
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
In Aortic Samples
Detected As An Extracellular Protein Loosely Bound To The Matrix
Alternative Antibody Names Anti-Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain antibody
Anti-CTSD antibody
Anti-CPSD antibody

Information sourced from Uniprot.org

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