• Western blot analysis of extracts of U2OS cells, using CTGF rabbit polyclonal antibody antibody (STJ113799) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using CTGF rabbit polyclonal antibody (STJ113799) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat kidney using CTGF rabbit polyclonal antibody (STJ113799) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human colon using CTGF rabbit polyclonal antibody (STJ113799) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 using CTGF antibody (STJ113799) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 using CTGF antibody (STJ113799) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CTGF antibody (100-200) (STJ113799)

SKU:
STJ113799

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CTGF (100-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCN2
Gene ID: 1490
Uniprot ID: CCN2_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CTGF (NP_001892.2).
Immunogen Sequence: GAPCIFGGTVYRSGESFQSS CKYQCTCLDGAVGCMPLCSM DVRLPSPDCPFPRRVKLPGK CCEEWVCDEPKDQTVVGPAL AAYRLEDTFGPDPTMIRANC L
Tissue Specificity Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
Function Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
Protein Name Ccn Family Member 2
Cellular Communication Network Factor 2
Connective Tissue Growth Factor
Hypertrophic Chondrocyte-Specific Protein 24
Insulin-Like Growth Factor-Binding Protein 8
Ibp-8
Igf-Binding Protein 8
Igfbp-8
Database Links Reactome: R-HSA-2032785
Reactome: R-HSA-8951671
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Ccn Family Member 2 antibody
Anti-Cellular Communication Network Factor 2 antibody
Anti-Connective Tissue Growth Factor antibody
Anti-Hypertrophic Chondrocyte-Specific Protein 24 antibody
Anti-Insulin-Like Growth Factor-Binding Protein 8 antibody
Anti-Ibp-8 antibody
Anti-Igf-Binding Protein 8 antibody
Anti-Igfbp-8 antibody
Anti-CCN2 antibody
Anti-CTGF antibody
Anti-HCS24 antibody
Anti-IGFBP8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance