• Western blot analysis of extracts of various cell lines, using Cystatin C antibody (STJ115255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.

Anti-CST3 antibody (27-146) (STJ115255)

SKU:
STJ115255

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CST3 (27-146) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CST3
Gene ID: 1471
Uniprot ID: CYTC_HUMAN
Immunogen Region: 27-146
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-146 of human Cystatin C (NP_000090.1).
Immunogen Sequence: SSPGKPPRLVGGPMDASVEE EGVRRALDFAVGEYNKASND MYHSRALQVVRARKQIVAGV NYFLDVELGRTTCTKTQPNL DNCPFHDQPHLKRKAFCSFQ IYAVPWQGTMTLSKSTCQDA
Tissue Specificity Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
Post Translational Modifications The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
Function As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Protein Name Cystatin-C
Cystatin-3
Gamma-Trace
Neuroendocrine Basic Polypeptide
Post-Gamma-Globulin
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-6798695
Reactome: R-HSA-8957275
Reactome: R-HSA-977225
Cellular Localisation Secreted
Alternative Antibody Names Anti-Cystatin-C antibody
Anti-Cystatin-3 antibody
Anti-Gamma-Trace antibody
Anti-Neuroendocrine Basic Polypeptide antibody
Anti-Post-Gamma-Globulin antibody
Anti-CST3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance