Anti-CSNK1A1 antibody (250-337) (STJ118665)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CSNK1A1 (250-337) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CSNK1A1 |
| Gene ID | 1452 |
| Uniprot ID | KC1A_HUMAN |
| Immunogen Region | 250-337 |
| Immunogen Sequence | EKKMSTPVEVLCKGFPAEFA MYLNYCRGLRFEEAPDYMYL RQLFRILFRTLNHQYDYTFD WTMLKQKAAQQAASSSGQGQ QAQTPTGKQTDKTKSNMKGF |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 238-337 of human Casein Kinase 1 alpha (CSNK1A1) (NP_001883.4). |
Additional Info
| Post Translational Modifications | Phosphorylated by MTOR in response to mitogenic stimulation, leading to its activation. |
| Function | Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration. Acts as a positive regulator of mTORC1 and mTORC2 signaling in response to nutrients by mediating phosphorylation of DEPTOR inhibitor. Acts as an inhibitor of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3. |
| Protein Name | Casein Kinase I Isoform AlphaCki-AlphaCk1 |
| Database Links | Reactome: R-HSA-195253Reactome: R-HSA-196299Reactome: R-HSA-4641262Reactome: R-HSA-5339716Reactome: R-HSA-5358747Reactome: R-HSA-5358749Reactome: R-HSA-5358751Reactome: R-HSA-5358752Reactome: R-HSA-5467337Reactome: R-HSA-5467340Reactome: R-HSA-5467348Reactome: R-HSA-5610783Reactome: R-HSA-5610785Reactome: R-HSA-5635838Reactome: R-HSA-9694631 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeChromosomeCentromereKinetochoreNucleus SpeckleCilium Basal BodySpindleLocalizes To The Centrosome In Interphase CellsAnd To Kinetochore Fibers During MitosisAlso Recruited To The Keratin Cytoskeleton |
| Alternative Antibody Names | Anti-Casein Kinase I Isoform Alpha antibodyAnti-Cki-Alpha antibodyAnti-Ck1 antibodyAnti-CSNK1A1 antibody |
Information sourced from Uniprot.org