• Immunoprecipitation analysis of 100 Mu g extracts of HeLa cells using 3 Mu g CSDE1 antibody (STJ27737). Western blot was performed from the immunoprecipitate using CSDE1 antibody (STJ27737) at a dilution of 1:1000.
  • Immunofluorescence analysis of L929 cells using CSDE1 Rabbit polyclonal antibody (STJ27737) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and CSDE1 knockout (KO) HeLa cells, using [KO Validated] CSDE1 Rabbit polyclonal antibody (STJ27737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-CSDE1 antibody (369-600) (STJ27737)

SKU:
STJ27737

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CSDE1 (369-600) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CSDE1
Gene ID: 7812
Uniprot ID: CSDE1_HUMAN
Immunogen Region: 369-600
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 369-600 of human CSDE1 (NP_009089.4).
Immunogen Sequence: LSAQRNHAIRIKKLPKGTVS FHSHSDHRFLGTVEKEATFS NPKTTSPNKGKEKEAEDGII AYDDCGVKLTIAFQAKDVEG STSPQIGDKVEFSISDKQRP GQQVATCVRLLGRNSNSKRL LGYVATLKDNFGFIETANHD KEIFFHYSEFSGDVDSLELG DMVEYSLSKGKGNKVSAEKV NKTHSVNGITEEADPTIYSG KVIRPLRSVDPTQTEYQGMI EIVEEGDMKGEV
Function RNA-binding protein involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Required for efficient formation of stress granules. (Microbial infection) Required for internal initiation of translation of human rhinovirus RNA.
Protein Name Cold Shock Domain-Containing Protein E1
N-Ras Upstream Gene Protein
Protein Unr
Cellular Localisation Cytoplasm
Stress Granule
P-Body
Alternative Antibody Names Anti-Cold Shock Domain-Containing Protein E1 antibody
Anti-N-Ras Upstream Gene Protein antibody
Anti-Protein Unr antibody
Anti-CSDE1 antibody
Anti-D1S155E antibody
Anti-KIAA0885 antibody
Anti-NRU antibody
Anti-UNR antibody

Information sourced from Uniprot.org

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