![Western blot analysis of lysates from wild type (WT) and CSDE1 knockout (KO) HeLa cells, using [KO Validated] CSDE1 Rabbit polyclonal antibody (STJ27737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101329/388954/STJ27737_3__92253.1713158535.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CSDE1 knockout (KO) HeLa cells, using [KO Validated] CSDE1 Rabbit polyclonal antibody (STJ27737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.")
| Host: | Rabbit |
| Applications: | WB/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CSDE1 |
| Gene ID: | 7812 |
| Uniprot ID: | CSDE1_HUMAN |
| Immunogen Region: | 369-600 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 369-600 of human CSDE1 (NP_009089.4). |
| Immunogen Sequence: | LSAQRNHAIRIKKLPKGTVS FHSHSDHRFLGTVEKEATFS NPKTTSPNKGKEKEAEDGII AYDDCGVKLTIAFQAKDVEG STSPQIGDKVEFSISDKQRP GQQVATCVRLLGRNSNSKRL LGYVATLKDNFGFIETANHD KEIFFHYSEFSGDVDSLELG DMVEYSLSKGKGNKVSAEKV NKTHSVNGITEEADPTIYSG KVIRPLRSVDPTQTEYQGMI EIVEEGDMKGEV |
| Function | RNA-binding protein involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Required for efficient formation of stress granules. (Microbial infection) Required for internal initiation of translation of human rhinovirus RNA. |
| Protein Name | Cold Shock Domain-Containing Protein E1N-Ras Upstream Gene ProteinProtein Unr |
| Cellular Localisation | CytoplasmStress GranuleP-Body |
| Alternative Antibody Names | Anti-Cold Shock Domain-Containing Protein E1 antibodyAnti-N-Ras Upstream Gene Protein antibodyAnti-Protein Unr antibodyAnti-CSDE1 antibodyAnti-D1S155E antibodyAnti-KIAA0885 antibodyAnti-NRU antibodyAnti-UNR antibody |
Information sourced from Uniprot.org