| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CS |
| Gene ID: | 1431 |
| Uniprot ID: | CISY_HUMAN |
| Immunogen Region: | 167-466 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 167-466 of human CS (NP_004068.2). |
| Immunogen Sequence: | QLSAAVTALNSESNFARAYA QGISRTKYWELIYEDSMDLI AKLPCVAAKIYRNLYREGSG IGAIDSNLDWSHNFTNMLGY TDHQFTELTRLYLTIHSDHE GGNVSAHTSHLVGSALSDPY LSFAAAMNGLAGPLHGLANQ EVLVWLTQLQKEVGKDVSDE KLRDYIWNTLNSGRVVPGYG HAVLRKTDPRYTCQREFALK HLPNDPMFKLVAQLYKIVPN VLLEQGKAKNPWPNVDAHS |
| Post Translational Modifications | Methylated. Trimethylation at Lys-395 by CSKMT decreases citrate synthase activity. In response to mitochondrial stress, the precursor protein is ubiquitinated by the SIFI complex in the cytoplasm before mitochondrial import, leading to its degradation. Within the SIFI complex, UBR4 initiates ubiquitin chain that are further elongated or branched by KCMF1. |
| Function | Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate. |
| Protein Name | Citrate Synthase - MitochondrialCitrateSi-Synthase |
| Database Links | Reactome: R-HSA-1268020Reactome: R-HSA-71403Reactome: R-HSA-9837999Reactome: R-HSA-9854311 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Citrate Synthase - Mitochondrial antibodyAnti-Citrate antibodyAnti-Si-Synthase antibodyAnti-CS antibody |
Information sourced from Uniprot.org