• Western blot analysis of extracts of various cell lines, using CRYAB rabbit monoclonal antibody (STJ11102511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of C6 cells using CRYAB Rabbit monoclonal antibody (STJ11102511) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat kidney using CRYAB rabbit monoclonal antibody (STJ11102511) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using CRYAB Rabbit monoclonal antibody (STJ11102511) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using CRYAB rabbit monoclonal antibody (STJ11102511) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CRYAB Rabbit monoclonal antibody (STJ11102511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CRYAB antibody (76-175) [S1MR] (STJ11102511)

SKU:
STJ11102511

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CRYAB (76-175) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRYAB
Gene ID: 1410
Uniprot ID: CRYAB_HUMAN
Immunogen Region: 76-175
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 76-175 of human CRYAB (P02511).
Immunogen Sequence: SVNLDVKHFSPEELKVKVLG DVIEVHGKHEERQDEHGFIS REFHRKYRIPADVDPLTITS SLSSDGVLTVNGPRKQVSGP ERTIPITREEKPAVTAAPKK
Tissue Specificity Lens as well as other tissues. Expressed in myocardial tissue.
Function May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome.
Protein Name Alpha-Crystallin B Chain
Alpha(B-Crystallin
Heat Shock Protein Beta-5
Hspb5
Renal Carcinoma Antigen Ny-Ren-27
Rosenthal Fiber Component
Database Links Reactome: R-HSA-3371571
Cellular Localisation Cytoplasm
Nucleus
Secreted
Lysosome
Translocates To The Nucleus During Heat Shock And Resides In Sub-Nuclear Structures Known As Sc35 Speckles Or Nuclear Splicing Speckles
Localizes At The Z-Bands And The Intercalated Disk In Cardiomyocytes
Can Be Secreted
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion
Alternative Antibody Names Anti-Alpha-Crystallin B Chain antibody
Anti-Alpha(B-Crystallin antibody
Anti-Heat Shock Protein Beta-5 antibody
Anti-Hspb5 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-27 antibody
Anti-Rosenthal Fiber Component antibody
Anti-CRYAB antibody
Anti-CRYA2 antibody
Anti-HSPB5 antibody

Information sourced from Uniprot.org

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