| Host: | Mouse |
| Applications: | WB/IP |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Crk II (C-Term) is suitable for use in Western Blot and Immunoprecipitation research applications. |
| Clonality : | Monoclonal |
| Clone ID : | M332 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Protein A Purified |
| Dilution Range: | WB 1:1000 IP 1:50 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Crk |
| Gene ID: | 12928 |
| Uniprot ID: | CRK_MOUSE |
| Immunogen Region: | C-Term |
| Specificity: | This antibody detects a 40 kDa* protein corresponding to the molecular mass of Crk II on SDS-PAGE immunoblots of rat PC12 and human Jurkat cells. |
| Immunogen: | Clone (M332) was generated from a recombinant sequence containing the C-terminal half of mouse Crk II. This sequence has high homology to human and rat Crk II. |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. Isoform Crk-II: Phosphorylated by KIT. Proline isomerization at Pro-237 by PPIA acts as a switch between two conformations: an autoinhibitory conformation in the cis form, where the tandem SH3 domains interact intramolecularly, and an activated conformation in the trans form. |
| Function | Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Isoform CRK-II: Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. |
| Protein Name | Adapter Molecule Crk Proto-Oncogene C-Crk P38 |
| Database Links | Reactome: R-MMU-170984 Reactome: -MMU-186763 Reactome: -MMU-2029482 Reactome: -MMU-372708 Reactome: -MMU-4420097 Reactome: -MMU-8849471 Reactome: -MMU-8875555 Reactome: -MMU-8875656 Reactome: -MMU-912631 |
| Cellular Localisation | Cytoplasm Cell Membrane Translocated To The Plasma Membrane Upon Cell Adhesion |
| Alternative Antibody Names | Anti-Adapter Molecule Crk antibody Anti-Proto-Oncogene C-Crk antibody Anti-P38 antibody Anti-Crk antibody Anti-Crko antibody |
Information sourced from Uniprot.org