• Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunofluorescence analysis of MCF7 cells, using CRHR2 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from COLO205 and RAW264. 7 cells, using CRHR2 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of the lysates from HepG2 cells using CRHR2 antibody.

Anti-CRHR2 antibody (71-120 aa) (STJ92475)

SKU:
STJ92475

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Corticotropin-releasing factor receptor 2 (71-120 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRHR2
Gene ID: 1395
Uniprot ID: CRFR2_HUMAN
Immunogen Region: 71-120 aa
Specificity: CRF-RII Polyclonal Antibody detects endogenous levels of CRF-RII protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CRHR2 at the amino acid range 71-120
Function G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels.
Protein Name Corticotropin-Releasing Factor Receptor 2
Crf-R-2
Crf-R2
Crfr-2
Corticotropin-Releasing Hormone Receptor 2
Crh-R-2
Crh-R2
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-418555
Reactome: R-HSA-422085
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Corticotropin-Releasing Factor Receptor 2 antibody
Anti-Crf-R-2 antibody
Anti-Crf-R2 antibody
Anti-Crfr-2 antibody
Anti-Corticotropin-Releasing Hormone Receptor 2 antibody
Anti-Crh-R-2 antibody
Anti-Crh-R2 antibody
Anti-CRHR2 antibody
Anti-CRF2R antibody
Anti-CRH2R antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance