• Immunofluorescence analysis of HeLa cells using CRHBP antibody (STJ28651). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CRHBP in paraffin-embedded rat kidney tissue using CRHBP Rabbit polyclonal antibody (STJ28651) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CRHBP in paraffin-embedded mouse kidney tissue using CRHBP Rabbit polyclonal antibody (STJ28651) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of Rat brain, using CRHBP Rabbit polyclonal antibody (STJ28651) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CRHBP antibody (25-322) (STJ28651)

SKU:
STJ28651

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CRHBP (25-322) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:100-1:500
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRHBP
Gene ID: 1393
Uniprot ID: CRHBP_HUMAN
Immunogen Region: 25-322
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-322 of human CRHBP (NP_001873.2).
Immunogen Sequence: YLELREAADYDPFLLFSANL KRELAGEQPYRRALRCLDML SLQGQFTFTADRPQLHCAAF FISEPEEFITIHYDQVSIDC QGGDFLKVFDGWILKGEKFP SSQDHPLPSAERYIDFCESG LSRRSIRSSQNVAMIFFRVH EPGNGFTLTIKTDPNLFPCN VISQTPNGKFTLVVPHQHRN CSFSIIYPVVIKISDLTLGH VNGLQLKKSSAGCEGIGDFV ELLGGTGLDPSKMTPLADL
Function Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy.
Protein Name Corticotropin-Releasing Factor-Binding Protein
Crf-Bp
Crf-Binding Protein
Corticotropin-Releasing Hormone-Binding Protein
Crh-Bp
Database Links Reactome: R-HSA-373080
Cellular Localisation Secreted
Alternative Antibody Names Anti-Corticotropin-Releasing Factor-Binding Protein antibody
Anti-Crf-Bp antibody
Anti-Crf-Binding Protein antibody
Anti-Corticotropin-Releasing Hormone-Binding Protein antibody
Anti-Crh-Bp antibody
Anti-CRHBP antibody
Anti-CRFBP antibody

Information sourced from Uniprot.org

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