| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CREB3L2 (1-247) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CREB3L2 |
| Gene ID: |
64764 |
| Uniprot ID: |
CR3L2_HUMAN |
| Immunogen Region: |
1-247 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-247 of human CREB3L2 (NP_001240704.1). |
| Immunogen Sequence: |
MEVLESGEQGVLQWDRKLSE LSEPGDGEALMYHTHFSELL DEFSQNVLGQLLNDPFLSEK SVSMEVEPSPTSPAPLIQAE HSYSLCEEPRAQSPFTHITT SDSFNDDEVESEKWYLSTDF PSTSIKTEPVTDEPPPGLVP SVTLTITAISTPLEKEEPPL EMNTGVDSSCQTIIPKIKLE PHEVDQFLNFSPKEGLSALP VSLWVMDMVSGSTEREYGER AGMSLYHRCCSWLYEIALF |
| Tissue Specificity | Widely expressed with highest levels in placenta, lung, spleen and intestine, and lowest levels in heart, brain, skeletal muscle, thymus, colon and leukocytes. In fetal tissues, the weakest expression is detected in brain and heart. |
| Post Translational Modifications | Upon ER stress, translocated to the Golgi apparatus, where it is processed by regulated intramembrane proteolysis (RIP) to release the cytosol-facing N-terminal transcription factor domain. The cleavage is performed sequentially by site-1 and site-2 proteases (S1P/MBTPS1 and S2P/MBTPS2). N-glycosylated. Ubiquitinated by HRD1/SYVN1.undergoes 'Lys-48'-linked ubiquitination, followed by rapid proteasomal degradation under normal conditions. Upon ER stress, SYVN1 E3 ubiquitin-protein ligase dissociates from its substrate, ubiquitination does not occur and CREB3L2 is stabilized. |
| Function | Transcription factor involved in unfolded protein response (UPR). In the absence of endoplasmic reticulum (ER) stress, inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. In response to ER stress, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. Plays a critical role in chondrogenesis by activating the transcription of SEC23A, which promotes the transport and secretion of cartilage matrix proteins, and possibly that of ER biogenesis-related genes. In a neuroblastoma cell line, protects cells from ER stress-induced death. In vitro activates transcription of target genes via direct binding to the CRE site. |
| Protein Name | Cyclic Amp-Responsive Element-Binding Protein 3-Like Protein 2Camp-Responsive Element-Binding Protein 3-Like Protein 2Bbf2 Human Homolog On Chromosome 7 Cleaved Into - Processed Cyclic Amp-Responsive Element-Binding Protein 3-Like Protein 2 |
| Database Links | Reactome: R-HSA-8874211 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane ProteinEr Membrane Resident ProteinUpon Er StressTranslocated To The Golgi Apparatus Where It Is CleavedThe Cytosolic N-Terminal Fragment (Processed Cyclic Amp-Responsive Element-Binding Protein 3-Like Protein 1) Is Transported Into The NucleusProcessed Cyclic Amp-Responsive Element-Binding Protein 3-Like Protein 2: NucleusTranslocated Into The Nucleus |
| Alternative Antibody Names | Anti-Cyclic Amp-Responsive Element-Binding Protein 3-Like Protein 2 antibodyAnti-Camp-Responsive Element-Binding Protein 3-Like Protein 2 antibodyAnti-Bbf2 Human Homolog On Chromosome 7 Cleaved Into - Processed Cyclic Amp-Responsive Element-Binding Protein 3-Like Protein 2 antibodyAnti-CREB3L2 antibodyAnti-BBF2H7 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
