Confocal imaging ofย paraffin-embedded Human tonsil tissue usingย CD35/CR1 Rabbit monoclonal antibody (STJ11101917, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-CR1 antibody (1940-2039) [S7MR] (STJ11101917)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11101917
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General Information

Short DescriptionRabbit monoclonal CD35 (1940-2039) antibody for use in IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS7MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeIHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCR1
Gene ID1378
Uniprot IDCR1_HUMAN
Immunogen Region1940-2039
Immunogen SequenceDGYTLEGSPWSQCQADDRWD PPLAKCTSRTHDALIVGTLS GTIFFILLIIFLSWIILKHR KGNNAHENPKEVAIHLHSQG GSSVHPRTLQTNEENSRVLP
SpecificityA synthetic peptide corresponding to a sequence within amino acids 1940-2039 of human CD35/CR1 (P17927).

Additional Info

Tissue Specificity Present on erythrocytes, a subset of T cells, mature B cells, follicular dendritic cells, monocytes and granulocytes.
Function Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages. Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation. Also acts in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d. Also plays a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells. (Microbial infection) Acts as a receptor for Epstein-Barr virus.
Protein Name Complement Receptor Type 1
C3b/C4b Receptor
Cd Antigen Cd35
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-8877330
Reactome: R-HSA-977606
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Complement Receptor Type 1 antibody
Anti-C3b/C4b Receptor antibody
Anti-Cd Antigen Cd35 antibody
Anti-CR1 antibody
Anti-C3BR antibody

Information sourced from Uniprot.org

Citations

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