• Immunohistochemistry analysis of CPT1B in paraffin-embedded mouse heart using CPT1B Rabbit polyclonal antibody (STJ28879) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CPT1B in paraffin-embedded human colon using CPT1B Rabbit polyclonal antibody (STJ28879) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat skeletal muscle, using CPT1B Rabbit polyclonal antibody (STJ28879) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CPT1B antibody (533-772) (STJ28879)

SKU:
STJ28879

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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CPT1B
Gene ID: 1375
Uniprot ID: CPT1B_HUMAN
Immunogen Region: 533-772
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 533-772 of human CPT1B (NP_004368.1).
Immunogen Sequence: SYQVAKALADDVELYCFQFL PFGKGLIKKCRTSPDAFVQI ALQLAHFRDRGKFCLTYEAS MTRMFREGRTETVRSCTSES TAFVQAMMEGSHTKADLRDL FQKAAKKHQNMYRLAMTGAG IDRHLFCLYLVSKYLGVSSP FLAEVLSEPWRLSTSQIPQS QIRMFDPEQHPNHLGAGGGF GPVADDGYGVSYMIAGENTI FFHISSKFSSSETNAQRFGN HIRKALLDIADLFQVPKAY
Tissue Specificity Strong expression in heart and skeletal muscle. No expression in liver and kidney.
Function Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion.
Protein Name Carnitine O-Palmitoyltransferase 1 - Muscle Isoform
Cpt1-M
Carnitine O-Palmitoyltransferase I - Muscle Isoform
Cpt I
Cpti-M
Carnitine Palmitoyltransferase 1b
Carnitine Palmitoyltransferase I-Like Protein
Database Links Reactome: R-HSA-200425
Reactome: R-HSA-5362517
Cellular Localisation Mitochondrion Outer Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Carnitine O-Palmitoyltransferase 1 - Muscle Isoform antibody
Anti-Cpt1-M antibody
Anti-Carnitine O-Palmitoyltransferase I - Muscle Isoform antibody
Anti-Cpt I antibody
Anti-Cpti-M antibody
Anti-Carnitine Palmitoyltransferase 1b antibody
Anti-Carnitine Palmitoyltransferase I-Like Protein antibody
Anti-CPT1B antibody
Anti-KIAA1670 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance