Host: | Rabbit |
Applications: | WB/IHC-P/ELISA |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Clonality: | Monoclonal |
Clone ID: | S3MR |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.01% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
Purification: | Affinity purification |
Concentration: | Lot specific |
Dilution Range: | WB:1:1000-1:6000 IHC-P:1:200-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | PTGS2 |
Gene ID: | 5743 |
Uniprot ID: | PGH2_HUMAN |
Immunogen Region: | 505-604 |
Specificity: | A synthetic peptide corresponding to a sequence within amino acids 505-604 of human COX2/PTGS2 (P35354). |
Immunogen Sequence: | GETMVEVGAPFSLKGLMGNV ICSPAYWKPSTFGGEVGFQI INTASIQSLICNNVKGCPFT SFSVPDPELIKTVTINASSS RSGLDDINPTVLLKERSTEL |
Post Translational Modifications | S-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-526. Acetylated at Ser-565 by SPHK1. During neuroinflammation, acetylation by SPHK1 promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia. |
Function | Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate, AA, C20:4(n-6)), with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate (DGLA, C20:3(n-6)) and eicosapentaenoate (EPA, C20:5(n-3)) to corresponding PGH1 and PGH3, the precursors of 1- and 3-series prostaglandins. In an alternative pathway of prostanoid biosynthesis, converts 2-arachidonoyl lysophopholipids to prostanoid lysophopholipids, which are then hydrolyzed by intracellular phospholipases to release free prostanoids. Metabolizes 2-arachidonoyl glycerol yielding the glyceryl ester of PGH2, a process that can contribute to pain response. Generates lipid mediators from n-3 and n-6 polyunsaturated fatty acids (PUFAs) via a lipoxygenase-type mechanism. Oxygenates PUFAs to hydroperoxy compounds and then reduces them to corresponding alcohols. Plays a role in the generation of resolution phase interaction products (resolvins) during both sterile and infectious inflammation. Metabolizes docosahexaenoate (DHA, C22:6(n-3)) to 17R-HDHA, a precursor of the D-series resolvins (RvDs). As a component of the biosynthetic pathway of E-series resolvins (RvEs), converts eicosapentaenoate (EPA, C20:5(n-3)) primarily to 18S-HEPE that is further metabolized by ALOX5 and LTA4H to generate 18S-RvE1 and 18S-RvE2. In vascular endothelial cells, converts docosapentaenoate (DPA, C22:5(n-3)) to 13R-HDPA, a precursor for 13-series resolvins (RvTs) shown to activate macrophage phagocytosis during bacterial infection. In activated leukocytes, contributes to oxygenation of hydroxyeicosatetraenoates (HETE) to diHETES (5,15-diHETE and 5,11-diHETE). Can also use linoleate (LA, (9Z,12Z)-octadecadienoate, C18:2(n-6)) as substrate and produce hydroxyoctadecadienoates (HODEs) in a regio- and stereospecific manner, being (9R)-HODE ((9R)-hydroxy-(10E,12Z)-octadecadienoate) and (13S)-HODE ((13S)-hydroxy-(9Z,11E)-octadecadienoate) its major products. During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs) 15R-lipoxin A4 that regulates phagocytic microglia. |
Protein Name | Prostaglandin G/H Synthase 2 Cyclooxygenase-2 Cox-2 Phs Ii Prostaglandin H2 Synthase 2 Pgh Synthase 2 Pghs-2 Prostaglandin-Endoperoxide Synthase 2 |
Database Links | Reactome: R-HSA-197264 Reactome: R-HSA-2142770 Reactome: R-HSA-2162123 Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-9018677 Reactome: R-HSA-9018679 Reactome: R-HSA-9025094 Reactome: R-HSA-9027604 |
Cellular Localisation | Microsome Membrane Peripheral Membrane Protein Endoplasmic Reticulum Membrane Nucleus Inner Membrane Nucleus Outer Membrane Detected On The Lumenal Side Of The Endoplasmic Reticulum And Nuclear Envelope |
Alternative Antibody Names | Anti-Prostaglandin G/H Synthase 2 antibody Anti-Cyclooxygenase-2 antibody Anti-Cox-2 antibody Anti-Phs Ii antibody Anti-Prostaglandin H2 Synthase 2 antibody Anti-Pgh Synthase 2 antibody Anti-Pghs-2 antibody Anti-Prostaglandin-Endoperoxide Synthase 2 antibody Anti-PTGS2 antibody Anti-COX2 antibody |
Information sourced from Uniprot.org