Anti-PTGS2 antibody (500-604 aa) [ABT-COX2] (STJ197010)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2bk
ImmunogenSynthesized peptide derived from the human COX-2 (cyclooxygenase-2) at the amino acid range 500-604
General Information
| Short Description | Mouse monoclonal anti-Prostaglandin G/H synthase 2 (500-604 aa) for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ABT-COX2 |
| Isotype | IgG2bk |
| Conjugation | Unconjugated |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 500-2000IHC-P 1:100-500 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PTGS2 |
| Gene ID | 5743 |
| Uniprot ID | PGH2_HUMAN |
| Immunogen | Synthesized peptide derived from the human COX-2 (cyclooxygenase-2) at the amino acid range 500-604 |
| Immunogen Region | 500-604 aa |
| Specificity | This antibody detects endogenous levels of human Prostaglandin G/H synthase 2 (EC 1.14.99.1) (Cyclooxygenase-2) (COX-2) (PHS II) (Prostaglandin H2 synthase 2) (PGH synthase 2) (PGHS-2) (Prostaglandin- |
Additional Info
| Function | Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate, AA, C20:4(n-6)), with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate (DGLA, C20:3(n-6)) and eicosapentaenoate (EPA, C20:5(n-3)) to corresponding PGH1 and PGH3, the precursors of 1- and 3-series prostaglandins. In an alternative pathway of prostanoid biosynthesis, converts 2-arachidonoyl lysophopholipids to prostanoid lysophopholipids, which are then hydrolyzed by intracellular phospholipases to release free prostanoids. Metabolizes 2-arachidonoyl glycerol yielding the glyceryl ester of PGH2, a process that can contribute to pain response. Generates lipid mediators from n-3 and n-6 polyunsaturated fatty acids (PUFAs) via a lipoxygenase-type mechanism. Oxygenates PUFAs to hydroperoxy compounds and then reduces them to corresponding alcohols. Plays a role in the generation of resolution phase interaction products (resolvins) during both sterile and infectious inflammation. Metabolizes docosahexaenoate (DHA, C22:6(n-3)) to 17R-HDHA, a precursor of the D-series resolvins (RvDs). As a component of the biosynthetic pathway of E-series resolvins (RvEs), converts eicosapentaenoate (EPA, C20:5(n-3)) primarily to 18S-HEPE that is further metabolized by ALOX5 and LTA4H to generate 18S-RvE1 and 18S-RvE2. In vascular endothelial cells, converts docosapentaenoate (DPA, C22:5(n-3)) to 13R-HDPA, a precursor for 13-series resolvins (RvTs) shown to activate macrophage phagocytosis during bacterial infection. In activated leukocytes, contributes to oxygenation of hydroxyeicosatetraenoates (HETE) to diHETES (5,15-diHETE and 5,11-diHETE). Can also use linoleate (LA, (9Z,12Z)-octadecadienoate, C18:2(n-6)) as substrate and produce hydroxyoctadecadienoates (HODEs) in a regio- and stereospecific manner, being (9R)-HODE ((9R)-hydroxy-(10E,12Z)-octadecadienoate) and (13S)-HODE ((13S)-hydroxy-(9Z,11E)-octadecadienoate) its major products. During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs) 15R-lipoxin A4 that regulates phagocytic microglia. |
| Protein Name | Prostaglandin G/H Synthase 2Cyclooxygenase-2Cox-2Phs IiProstaglandin H2 Synthase 2Pgh Synthase 2Pghs-2Prostaglandin-Endoperoxide Synthase 2 |
| Database Links | Reactome: R-HSA-197264Reactome: R-HSA-2142770Reactome: R-HSA-2162123Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-9018677Reactome: R-HSA-9018679Reactome: R-HSA-9025094Reactome: R-HSA-9027604 |
| Cellular Localisation | Microsome MembranePeripheral Membrane ProteinEndoplasmic Reticulum MembraneNucleus Inner MembraneNucleus Outer MembraneDetected On The Lumenal Side Of The Endoplasmic Reticulum And Nuclear Envelope |
| Alternative Antibody Names | Anti-Prostaglandin G/H Synthase 2 antibodyAnti-Cyclooxygenase-2 antibodyAnti-Cox-2 antibodyAnti-Phs Ii antibodyAnti-Prostaglandin H2 Synthase 2 antibodyAnti-Pgh Synthase 2 antibodyAnti-Pghs-2 antibodyAnti-Prostaglandin-Endoperoxide Synthase 2 antibodyAnti-PTGS2 antibodyAnti-COX2 antibody |
Information sourced from Uniprot.org