||WB, IHC, IF, IP
||Human, Mouse, Rat
||Rabbit polyclonal anti-Cox2 antibody is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
||FOR RESEARCH USE ONLY (RUO).
||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
||Store at-20°C. Avoid freeze/thaw cycles.
||WB: 1:1000-1:4000IHC: 1:50-1:100IF: 1:50-1:100IP: 1:50-1:100
||Recombinant fusion protein containing a sequence corresponding to amino acids 375-604 of human Cox2 (NP_000954.1).
| Post Translational Modifications || S-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-526. Acetylated at Ser-565 by SPHK1. During neuroinflammation, acetylation by SPHK1 promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia. |
| Function || Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate (DGLA, C20:3(n-6)) and eicosapentaenoate (EPA, C20:5(n-3)) to corresponding PGH1 and PGH3, the precursors of 1- and 3-series prostaglandins. In an alternative pathway of prostanoid biosynthesis, converts 2-arachidonoyl lysophopholipids to prostanoid lysophopholipids, which are then hydrolyzed by intracellular phospholipases to release free prostanoids. Metabolizes 2-arachidonoyl glycerol yielding the glyceryl ester of PGH2, a process that can contribute to pain response. Generates lipid mediators from n-3 and n-6 polyunsaturated fatty acids (PUFAs) via a lipoxygenase-type mechanism. Oxygenates PUFAs to hydroperoxy compounds and then reduces them to corresponding alcohols. Plays a role in the generation of resolution phase interaction products (resolvins) during both sterile and infectious inflammation. Metabolizes docosahexaenoate (DHA, C22:6(n-3)) to 17R-HDHA, a precursor of the D-series resolvins (RvDs). As a component of the biosynthetic pathway of E-series resolvins (RvEs), converts eicosapentaenoate (EPA, C20:5(n-3)) primarily to 18S-HEPE that is further metabolized by ALOX5 and LTA4H to generate 18S-RvE1 and 18S-RvE2. In vascular endothelial cells, converts docosapentaenoate (DPA, C22:5(n-3)) to 13R-HDPA, a precursor for 13-series resolvins (RvTs) shown to activate macrophage phagocytosis during bacterial infection. In activated leukocytes, contributes to oxygenation of hydroxyeicosatetraenoates (HETE) to diHETES (5,15-diHETE and 5,11-diHETE). During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs) 15R-lipoxin A4 that regulates phagocytic microglia. |
| Protein Name || Prostaglandin G/H Synthase 2Cyclooxygenase-2Cox-2Phs IiProstaglandin H2 Synthase 2Pgh Synthase 2Pghs-2Prostaglandin-Endoperoxide Synthase 2 |
| Database Links || Reactome: R-HSA-197264Reactome: R-HSA-2142770Reactome: R-HSA-2162123Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-9018677Reactome: R-HSA-9018679Reactome: R-HSA-9025094Reactome: R-HSA-9027604 |
| Cellular Localisation || Microsome MembranePeripheral Membrane ProteinEndoplasmic Reticulum MembraneNucleus Inner MembraneNucleus Outer MembraneDetected On The Lumenal Side Of The Endoplasmic Reticulum And Nuclear Envelope |
| Alternative Antibody Names || Anti-Prostaglandin G/H Synthase 2 antibodyAnti-Cyclooxygenase-2 antibodyAnti-Cox-2 antibodyAnti-Phs Ii antibodyAnti-Prostaglandin H2 Synthase 2 antibodyAnti-Pgh Synthase 2 antibodyAnti-Pghs-2 antibodyAnti-Prostaglandin-Endoperoxide Synthase 2 antibodyAnti-PTGS2 antibodyAnti-COX2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance