• Immunofluorescence analysis of HeLa cells using Cox2 antibody (STJ111060) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human liver cancer using Cox2 antibody (STJ111060) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse spleen using Cox2 antibody (STJ111060) at dilution of 1:200 (40x lens).
  • Western blot analysis of extracts of various cell lines, using Cox2 Rabbit pAb (STJ111060) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

Anti-Cox2 antibody (STJ111060)

SKU:
STJ111060

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Host: Rabbit
Applications: WB, IHC, IF, IP
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-Cox2 antibody is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:1000-1:4000
IHC: 1:50-1:100
IF: 1:50-1:100
IP: 1:50-1:100
Uniprot ID: PGH2_HUMAN
Gene ID: 5743
Gene Symbol: PTGS2
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 375-604 of human Cox2 (NP_000954.1).
Post Translational Modifications S-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-526. Acetylated at Ser-565 by SPHK1. During neuroinflammation, acetylation by SPHK1 promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.
Function Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate (DGLA, C20:3(n-6)) and eicosapentaenoate (EPA, C20:5(n-3)) to corresponding PGH1 and PGH3, the precursors of 1- and 3-series prostaglandins. In an alternative pathway of prostanoid biosynthesis, converts 2-arachidonoyl lysophopholipids to prostanoid lysophopholipids, which are then hydrolyzed by intracellular phospholipases to release free prostanoids. Metabolizes 2-arachidonoyl glycerol yielding the glyceryl ester of PGH2, a process that can contribute to pain response. Generates lipid mediators from n-3 and n-6 polyunsaturated fatty acids (PUFAs) via a lipoxygenase-type mechanism. Oxygenates PUFAs to hydroperoxy compounds and then reduces them to corresponding alcohols. Plays a role in the generation of resolution phase interaction products (resolvins) during both sterile and infectious inflammation. Metabolizes docosahexaenoate (DHA, C22:6(n-3)) to 17R-HDHA, a precursor of the D-series resolvins (RvDs). As a component of the biosynthetic pathway of E-series resolvins (RvEs), converts eicosapentaenoate (EPA, C20:5(n-3)) primarily to 18S-HEPE that is further metabolized by ALOX5 and LTA4H to generate 18S-RvE1 and 18S-RvE2. In vascular endothelial cells, converts docosapentaenoate (DPA, C22:5(n-3)) to 13R-HDPA, a precursor for 13-series resolvins (RvTs) shown to activate macrophage phagocytosis during bacterial infection. In activated leukocytes, contributes to oxygenation of hydroxyeicosatetraenoates (HETE) to diHETES (5,15-diHETE and 5,11-diHETE). During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs) 15R-lipoxin A4 that regulates phagocytic microglia.
Protein Name Prostaglandin G/H Synthase 2
Cyclooxygenase-2
Cox-2
Phs Ii
Prostaglandin H2 Synthase 2
Pgh Synthase 2
Pghs-2
Prostaglandin-Endoperoxide Synthase 2
Database Links Reactome: R-HSA-197264
Reactome: R-HSA-2142770
Reactome: R-HSA-2162123
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-9018677
Reactome: R-HSA-9018679
Reactome: R-HSA-9025094
Reactome: R-HSA-9027604
Cellular Localisation Microsome Membrane
Peripheral Membrane Protein
Endoplasmic Reticulum Membrane
Nucleus Inner Membrane
Nucleus Outer Membrane
Detected On The Lumenal Side Of The Endoplasmic Reticulum And Nuclear Envelope
Alternative Antibody Names Anti-Prostaglandin G/H Synthase 2 antibody
Anti-Cyclooxygenase-2 antibody
Anti-Cox-2 antibody
Anti-Phs Ii antibody
Anti-Prostaglandin H2 Synthase 2 antibody
Anti-Pgh Synthase 2 antibody
Anti-Pghs-2 antibody
Anti-Prostaglandin-Endoperoxide Synthase 2 antibody
Anti-PTGS2 antibody
Anti-COX2 antibody

Information sourced from Uniprot.org

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