| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | COQ3 |
| Gene ID: | 51805 |
| Uniprot ID: | COQ3_HUMAN |
| Immunogen Region: | 50-369 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-369 of human COQ3 (NP_059117.3). |
| Immunogen Sequence: | PGVFNEYRTIWFKSYRTIFS CLNRIKSFRYPWARLYSTSQ TTVDSGEVKTFLALAHKWWD EQGVYAPLHSMNDLRVPFIR DNLLKTIPNHQPGKPLLGMK ILDVGCGGGLLTEPLGRLGA SVIGIDPVDENIKTAQCHKS FDPVLDKRIEYRVCSLEEIV EETAETFDAVVASEVVEHVI DLETFLQCCCQVLKPGGSLF ITTINKTQLSYALGIVFSEQ IASIVPKGTHTWEKFVSPE |
| Function | O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway. |
| Protein Name | Ubiquinone Biosynthesis O-Methyltransferase - Mitochondrial3-Demethylubiquinol 3-O-MethyltransferasePolyprenyldihydroxybenzoate Methyltransferase |
| Database Links | Reactome: R-HSA-2142789 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinMatrix Side |
| Alternative Antibody Names | Anti-Ubiquinone Biosynthesis O-Methyltransferase - Mitochondrial antibodyAnti-3-Demethylubiquinol 3-O-Methyltransferase antibodyAnti-Polyprenyldihydroxybenzoate Methyltransferase antibodyAnti-COQ3 antibodyAnti-UG0215E05 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and TWF2 knockout (KO) 293T cells, using [KO Validated] TWF2 Rabbit polyclonal antibody (STJ11100774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89710/358192/STJ11100774_1__12392.1713122508.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TWF2 knockout (KO) 293T cells, using [KO Validated] TWF2 Rabbit polyclonal antibody (STJ11100774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
