• Chromatin immunoprecipitation analysis of extracts of HeLa cells, using JAB1/CSN5/COPS5 antibody (STJ11101993) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunofluorescence analysis of U-2 OS cells using JAB1/CSN5/COPS5 Rabbit monoclonal antibody (STJ11101993) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using JAB1/CSN5/COPS5 Rabbit monoclonal antibody (STJ11101993) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of JAB1/CSN5/COPS5 in paraffin-embedded mouse colon tissue using JAB1/CSN5/COPS5 Rabbit monoclonal antibody (STJ11101993) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of JAB1/CSN5/COPS5 in paraffin-embedded rat testis tissue using JAB1/CSN5/COPS5 Rabbit monoclonal antibody (STJ11101993) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of JAB1/CSN5/COPS5 in paraffin-embedded mouse testis tissue using JAB1/CSN5/COPS5 Rabbit monoclonal antibody (STJ11101993) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of JAB1/CSN5/COPS5 in paraffin-embedded rat colon tissue using JAB1/CSN5/COPS5 Rabbit monoclonal antibody (STJ11101993) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of JAB1/CSN5/COPS5 in paraffin-embedded human breast tissue using JAB1/CSN5/COPS5 Rabbit monoclonal antibody (STJ11101993) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of JAB1/CSN5/COPS5 in paraffin-embedded human liver cancer tissue using JAB1/CSN5/COPS5 Rabbit monoclonal antibody (STJ11101993) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using JAB1/CSN5/COPS5 Rabbit monoclonal antibody (STJ11101993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-COPS5 antibody (1-100) [S3MR] (STJ11101993)

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STJ11101993

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-JAB1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COPS5
Gene ID: 10987
Uniprot ID: CSN5_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human JAB1/CSN5/COPS5 (Q92905).
Immunogen Sequence: MAASGSGMAQKTWELANNMQ EAQSIDEIYKYDKKQQQEIL AAKPWTKDHHYFKYCKISAL ALLKMVMHARSGGNLEVMGL MLGKVDGETMIIMDSFALPV
Function Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2.
Protein Name Cop9 Signalosome Complex Subunit 5
Sgn5
Signalosome Subunit 5
Jun Activation Domain-Binding Protein 1
Database Links Reactome: R-HSA-5696394
Reactome: R-HSA-6781823
Reactome: R-HSA-8856825
Reactome: R-HSA-8951664
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Perinuclear Region
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle
Nuclear Localization Is Diminished In The Presence Of Ifit3
Alternative Antibody Names Anti-Cop9 Signalosome Complex Subunit 5 antibody
Anti-Sgn5 antibody
Anti-Signalosome Subunit 5 antibody
Anti-Jun Activation Domain-Binding Protein 1 antibody
Anti-COPS5 antibody
Anti-CSN5 antibody
Anti-JAB1 antibody

Information sourced from Uniprot.org

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