• STJ70095 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70095 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-COP1/PSEUDO-ICE-Isoform 2 antibody (C-Term) (STJ70095)

SKU:
STJ70095-100

£312.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-COP1/PSEUDO-ICE-Isoform 2 (C-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:4000.
IF: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/ml.

FC:Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CARD16
Gene ID: 114769
Uniprot ID: CAR16_HUMAN
Immunogen Region: C-Term
Accession Number: NP_443121.1
Specificity: This antibody is expected to recognize isoform 2 (NP_443121.1) only. In addition there may be cross-reaction with INCA (GeneID 440068; NP_001007233.1).
Immunogen Sequence: ETLGLSAGPIPGN
Function Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokine response. Also able to inhibit CASP1-mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2.
Protein Name Caspase Recruitment Domain-Containing Protein 16
Caspase Recruitment Domain-Only Protein 1
Card-Only Protein 1
Caspase-1 Inhibitor Cop
Pseudo Interleukin-1 Beta Converting Enzyme
Pseudo-Ice
Pseudo-Il1b-Converting Enzyme
Alternative Antibody Names Anti-Caspase Recruitment Domain-Containing Protein 16 antibody
Anti-Caspase Recruitment Domain-Only Protein 1 antibody
Anti-Card-Only Protein 1 antibody
Anti-Caspase-1 Inhibitor Cop antibody
Anti-Pseudo Interleukin-1 Beta Converting Enzyme antibody
Anti-Pseudo-Ice antibody
Anti-Pseudo-Il1b-Converting Enzyme antibody
Anti-CARD16 antibody
Anti-COP antibody
Anti-COP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance