| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-COMT (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 28kDa band in Human Testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1). Recommended concentration: 0.03-0.1µg/ml.IHC: I |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | COMT |
| Gene ID: | 1312 |
| Uniprot ID: | COMT_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_000745.1; NP_009294.1 |
| Specificity: | This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1) |
| Immunogen Sequence: | QDIIPQLKKKYDVD |
| Post Translational Modifications | The N-terminus is blocked. |
| Function | Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. |
| Protein Name | Catechol O-Methyltransferase |
| Database Links | Reactome: R-HSA-156581Reactome: R-HSA-379397Reactome: R-HSA-379398Reactome: R-HSA-9679191 |
| Cellular Localisation | Isoform Soluble: CytoplasmIsoform Membrane-Bound: Cell MembraneSingle-Pass Type Ii Membrane ProteinExtracellular Side |
| Alternative Antibody Names | Anti-Catechol O-Methyltransferase antibodyAnti-COMT antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from THP-1 cells using [KD Validated] COMT Rabbit pAb (STJ11106950) at 1:700 dilution. <br/>Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) ( STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/186146/407849/STJ11106950_1__05562.1744894339.jpg?c=1 "Western blot analysis of lysates from THP-1 cells using [KD Validated] COMT Rabbit pAb (STJ11106950) at 1:700 dilution. <br/>Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) ( STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.")
