• STJ70740 staining (0. 03µg/ml) of Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70740 staining (0. 3µg/ml) of K562 cell lysate (35µg protein in RIPA buffer) Detected by chemiluminescence.
  • STJ70740 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • STJ70740 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Complement factor H antibody (Internal) (STJ70740)

SKU:
STJ70740-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Complement factor H (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.03-0.3µg/ml
IHC-5µg/ml
FC-Flow cytometric analysis of HepG2 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CFH
Gene ID: 3075
Uniprot ID: CFAH_HUMAN
Immunogen Region: Internal
Accession Number: NP_000177.2
Specificity: This antibody is expected to recognize isoform a (NP_000177.2) only.
Immunogen Sequence: HLVPDRKKDQYK
Post Translational Modifications Sulfated on tyrosine residues.
Function Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed. (Microbial infection) In the mosquito midgut, binds to the surface of parasite P.falciparum gametocytes and protects the parasite from alternative complement pathway-mediated elimination.
Protein Name Complement Factor H
H Factor 1
Database Links Reactome: R-HSA-977606
Cellular Localisation Secreted
(Microbial Infection) In The Mosquito Midgut
Localizes To P
Falciparum (Nf54 Strain) Macrogamete And Young Zygote Cell Membranes
Alternative Antibody Names Anti-Complement Factor H antibody
Anti-H Factor 1 antibody
Anti-CFH antibody
Anti-HF antibody
Anti-HF1 antibody
Anti-HF2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance