• Western blot analysis of extracts of various cell lines, using Collagen VI/Collagen VI/COL6A1 antibody (STJ111622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using Collagen VI/Collagen VI/COL6A1 antibody (STJ111622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-COL6A1 antibody (19-250) (STJ111622)

SKU:
STJ111622

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COL6A1 (19-250) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL6A1
Gene ID: 1291
Uniprot ID: CO6A1_HUMAN
Immunogen Region: 19-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-250 of human Collagen VI/Collagen VI/COL6A1 (NP_001839.2).
Immunogen Sequence: AQDEPETPRAVAFQDCPVDL FFVLDTSESVALRLKPYGAL VDKVKSFTKRFIDNLRDRYY RCDRNLVWNAGALHYSDEVE IIQGLTRMPGGRDALKSSVD AVKYFGKGTYTDCAIKKGLE QLLVGGSHLKENKYLIVVTD GHPLEGYKEPCGGLEDAVNE AKHLGVKVFSVAITPDHLEP RLSIIATDHTYRRNFTAADW GQSRDAEEAISQTIDTIVDM IKNNVEQVCCSF
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Function Collagen VI acts as a cell-binding protein.
Protein Name Collagen Alpha-1(Vi Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1650814
Reactome: R-HSA-186797
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000178
Reactome: R-HSA-419037
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-1(Vi Chain antibody
Anti-COL6A1 antibody

Information sourced from Uniprot.org

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