• Western blot analysis of Saos-2, using COL5A1 Rabbit polyclonal antibody (STJ23194) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-COL5A1 antibody (300-400) (STJ23194)

SKU:
STJ23194

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COL5A1 (300-400) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL5A1
Gene ID: 1289
Uniprot ID: CO5A1_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human COL5A1 (NP_000084.3).
Immunogen Sequence: AKETTEVPEELTPTPTEAAP MPETSEGAGKEEDVGIGDYD YVPSEDYYTPSPYDDLTYGE GEENPDQPTDPGAGAEIPTS TADTSNSSNPAPPPGEGADD L
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Sulfated on 40% of tyrosines.
Function Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
Protein Name Collagen Alpha-1(V Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474244
Reactome: R-HSA-1650814
Reactome: R-HSA-186797
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000170
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-419037
Reactome: R-HSA-8874081
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-1(V Chain antibody
Anti-COL5A1 antibody

Information sourced from Uniprot.org

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