Western blot analysis of various lysates using COL4A5 Rabbit pAb (STJ111851) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-COL4A5 antibody (1462-1691) (STJ111851)

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STJ111851
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Short Description :Rabbit polyclonal COL4A5 (1462-1691) antibody for use in WB, IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/IF/ICC/ELISA
Host:Rabbit
Reactivity:Human
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:500-1:2000
IF/ICC:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:COL4A5
Gene ID:1287
Uniprot ID:CO4A5_HUMAN
Immunogen Region:1462-1691
Immunogen Sequence:SSVAHGFLITRHSQTTDAPQ CPQGTLQVYEGFSLLYVQGN KRAHGQDLGTAGSCLRRFST MPFMFCNINNVCNFASRNDY SYWLSTPEPMPMSMQPLKGQ SIQPFISRCAVCEAPAVVIA VHSQTIQIPHCPQGWDSLWI GYSFMMHTSAGAEGSGQALA SPGSCLEEFRSAPFIECHGR GTCNYYANSYSFWLATVDVS DMFSKPQSETLKAGDLRTRI SRCQVCMKRT
Specificity:Recombinant fusion protein containing a sequence corresponding to amino acids 1462-1691 of human COL4A5 (NP_203699.1).
Tissue Specificity Isoform 2 is found in kidney.
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Protein Name Collagen Alpha-5(Iv Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474244
Reactome: R-HSA-1650814
Reactome: R-HSA-186797
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-2214320
Reactome: R-HSA-2243919
Reactome: R-HSA-3000157
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-419037
Reactome: R-HSA-8948216
Reactome: R-HSA-9010553
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Alternative Antibody Names Anti-Collagen Alpha-5(Iv Chain antibody
Anti-COL4A5 antibody

Information sourced from Uniprot.org

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