• Western blot analysis of extracts of various cell lines, using CNTN4 antibody (STJ112377) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CNTN4 antibody (448-697) (STJ112377)

SKU:
STJ112377

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CNTN4 (448-697) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CNTN4
Gene ID: 152330
Uniprot ID: CNTN4_HUMAN
Immunogen Region: 448-697
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 448-697 of human CNTN4 (NP_001193885.1).
Immunogen Sequence: VGVFNNKGEGPFSPTTVVYS AEEEPTKPPASIFARSLSAT DIEVFWASPLEKNRGRIQGY EVKYWRHEDKEENARKIRTV GNQTSTKITNLKGSVLYHLA VKAYNSAGTGPSSATVNVTT RKPPPSQPPGNIIWNSSDSK IILNWDQVKALDNESEVKGY KVLYRWNRQSSTSVIETNKT SVELSLPFDEDYIIEIKPFS DGGDGSSSEQIRIPKISNAY ARGSGASTSNACTLSAIST
Tissue Specificity Mainly expressed in brain. Highly expressed in cerebellum and weakly expressed in corpus callosum, caudate nucleus, amygdala and spinal cord. Also expressed in testis, pancreas, thyroid, uterus, small intestine and kidney. Not expressed in skeletal muscle. Isoform 2 is weakly expressed in cerebral cortex.
Function Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.
Protein Name Contactin-4
Brain-Derived Immunoglobulin Superfamily Protein 2
Big-2
Database Links Reactome: R-HSA-163125
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Secreted
Alternative Antibody Names Anti-Contactin-4 antibody
Anti-Brain-Derived Immunoglobulin Superfamily Protein 2 antibody
Anti-Big-2 antibody
Anti-CNTN4 antibody

Information sourced from Uniprot.org

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