• Confocal imaging of paraffin-embedded Mouse small intestine tissue using Calponin Rabbit monoclonal antibody (STJ11101941, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat small intestine tissue using Calponin Rabbit monoclonal antibody (STJ11101941, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of Calponin in paraffin-embedded human colon using Calponin Rabbit monoclonal antibody (STJ11101941) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Calponin Rabbit monoclonal antibody (STJ11101941) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CNN1 antibody (198-297) [S1MR] (STJ11101941)

SKU:
STJ11101941

£146.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IHC-P:1:1000-1:5000
IF/ICC:1:200-1:800
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CNN1
Gene ID: 1264
Uniprot ID: CNN1_HUMAN
Immunogen Region: 198-297
Specificity: A synthetic peptide corresponding to a sequence within amino acids 198-297 of human Calponin (P51911).
Immunogen Sequence: PLDQATISLQMGTNKGASQA GMTAPGTKRQIFEPGLGMEH CDTLNVSLQMGSNKGASQRG MTVYGLPRQVYDPKYCLTPE YPELGEPAHNHHAHNYYNSA
Tissue Specificity Smooth muscle, and tissues containing significant amounts of smooth muscle.
Function Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Protein Name Calponin-1
Basic Calponin
Calponin H1 - Smooth Muscle
Alternative Antibody Names Anti-Calponin-1 antibody
Anti-Basic Calponin antibody
Anti-Calponin H1 - Smooth Muscle antibody
Anti-CNN1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance