• Immunofluorescence analysis of U-2 OS cells using CNBP Rabbit polyclonal antibody (STJ117304) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CNBP in paraffin-embedded Rat colon tissue using CNBP Rabbit polyclonal antibody (STJ117304) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CNBP in paraffin-embedded Mouse colon tissue using CNBP Rabbit polyclonal antibody (STJ117304) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CNBP in paraffin-embedded Human liver tissue using CNBP Rabbit polyclonal antibody (STJ117304) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CNBP in paraffin-embedded Human kidney tissue using CNBP Rabbit polyclonal antibody (STJ117304) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates, using CNBP Rabbit polyclonal antibody (STJ117304) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CNBP antibody (70-170) (STJ117304)

SKU:
STJ117304

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CNBP (70-170) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CNBP
Gene ID: 7555
Uniprot ID: CNBP_HUMAN
Immunogen Region: 70-170
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 70-170 of human CNBP (NP_001120668.1).
Immunogen Sequence: CGRGGHIAKDCKEPKREREQ CCYNCGKPGHLARDCDHADE QKCYSCGEFGHIQKDCTKVK CYRCGETGHVAINCSKTSEV NCYRCGESGHLARECTIEAT A
Tissue Specificity Expressed in the liver, kidney, spleen, testis, lung, muscle and adrenal glands.
Post Translational Modifications Arginine methylation by PRMT1 in the Arg/Gly-rich region impedes RNA binding.
Function Single-stranded DNA-binding protein that preferentially binds to the sterol regulatory element (SRE) sequence 5'-GTGCGGTG-3', and thereby mediates transcriptional repression. Has a role as transactivator of the Myc promoter. Binds single-stranded RNA in a sequence-specific manner. Isoform 1: Binds G-rich elements in target mRNA coding sequences. Prevents G-quadruplex structure formation in vitro, suggesting a role in supporting translation by resolving stable structures on mRNAs. Isoform 2: Binds to RNA. Isoform 4: Binds to RNA. Isoform 5: Binds to RNA. Isoform 6: Binds to RNA. Isoform 8: Binds to RNA.
Protein Name Cchc-Type Zinc Finger Nucleic Acid Binding Protein
Cellular Nucleic Acid-Binding Protein
Cnbp
Zinc Finger Protein 9
Database Links Reactome: R-HSA-9705671
Cellular Localisation Nucleus
Cytoplasm
Endoplasmic Reticulum
Isoform 1: Cytoplasm
Isoform 2: Cytoplasm
Isoform 4: Cytoplasm
Isoform 5: Cytoplasm
Isoform 6: Cytoplasm
Isoform 8: Cytoplasm
Alternative Antibody Names Anti-Cchc-Type Zinc Finger Nucleic Acid Binding Protein antibody
Anti-Cellular Nucleic Acid-Binding Protein antibody
Anti-Cnbp antibody
Anti-Zinc Finger Protein 9 antibody
Anti-CNBP antibody
Anti-RNF163 antibody
Anti-ZNF9 antibody

Information sourced from Uniprot.org

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