• Western blot analysis of extracts of various cell lines, using CLTA antibody (STJ23172) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CLTA antibody (1-218) (STJ23172)

SKU:
STJ23172

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLTA (1-218) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLTA
Gene ID: 1211
Uniprot ID: CLCA_HUMAN
Immunogen Region: 1-218
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human CLTA (NP_001824.1).
Immunogen Sequence: MAELDPFGAPAGAPGGPALG NGVAGAGEEDPAAAFLAQQE SEIAGIENDEAFAILDGGAP GPQPHGEPPGGPDAVDGVMN GEYYQESNGPTDSYAAISQV DRLQSEPESIRKWREEQMER LEALDANSRKQEAEWKEKAI KELEEWYARQDEQLQKTKAN NRAAEEAFVNDIDESSPGTE WERVARLCDFNPKSSKQAKD VSRMRSVLISLKQAPLVH
Function Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge.
Protein Name Clathrin Light Chain A
Lca
Database Links Reactome: R-HSA-168275
Reactome: R-HSA-177504
Reactome: R-HSA-190873
Reactome: R-HSA-196025
Reactome: R-HSA-2132295
Reactome: R-HSA-3928665
Reactome: R-HSA-432720
Reactome: R-HSA-432722 P09496-1
Reactome: R-HSA-437239
Reactome: R-HSA-5099900
Reactome: R-HSA-5140745
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8866427
Reactome: R-HSA-8964038
Cellular Localisation Cytoplasmic Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Membrane
Coated Pit
Cytoplasm
Cytoskeleton
Spindle
Cytoplasmic Face Of Coated Pits And Vesicles
In Complex With Tacc3 And Ckap5 (Forming The Tacc3/Ch-Tog/Clathrin Complex) Localized To Inter-Microtubule Bridges In Mitotic Spindles
Alternative Antibody Names Anti-Clathrin Light Chain A antibody
Anti-Lca antibody
Anti-CLTA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance