• Immunoprecipitation analysis of 300 Mu g extracts of Hela cells using 3 Mu g [KD Validated] CLIC1 Rabbit polyclonal antibody (STJ11105200). Western blot was performed from the immunoprecipitate using [KO Validated] CLIC1 Rabbit polyclonal antibody (STJ11105200) at a dilition of 1:2000.
  • Immunofluorescence analysis of THP-1 cells using [KD Validated] CLIC1 Rabbit polyclonal antibody (STJ11105200) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of K-562 cells using [KD Validated] CLIC1 Rabbit polyclonal antibody (STJ11105200) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and CLIC1 knockdown (KD) HeLa cells, using [KD Validated] CLIC1 Rabbit polyclonal antibody (STJ11105200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of lysates from MCF7 cells, using [KD Validated] CLIC1 Rabbit polyclonal antibody (STJ11105200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CLIC1 antibody (42-241) (STJ11105200)

SKU:
STJ11105200

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Chloride Intracellular Channel Protein 1 (42-241) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLIC1
Gene ID: 1192
Uniprot ID: CLIC1_HUMAN
Immunogen Region: 42-241
Immunogen: Recombinant Protein corresponding to a sequence within amino acids 42-241 of human CLIC1 (NP_001279.2).
Immunogen Sequence: NVTTVDTKRRTETVQKLCPG GQLPFLLYGTEVHTDTNKIE EFLEAVLCPPRYPKLAALNP ESNTAGLDIFAKFSAYIKNS NPALNDNLEKGLLKALKVLD NYLTSPLPEEVDETSAEDEG VSQRKFLDGNELTLADCNLL PKLHIVQVVCKKYRGFTIPE AFRGVHRYLSNAYAREEFAS TCPDDEEIELAYEQVAKALK
Tissue Specificity Expression is prominent in heart, placenta, liver, kidney and pancreas.
Post Translational Modifications Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.
Function Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
Protein Name Chloride Intracellular Channel Protein 1
Chloride Channel Abp
Nuclear Chloride Ion Channel 27
Ncc27
Regulatory Nuclear Chloride Ion Channel Protein
Hrncc
Cellular Localisation Nucleus
Nucleus Membrane
Single-Pass Membrane Protein
Cytoplasm
Cell Membrane
Endoplasmic Reticulum
Mostly In The Nucleus Including In The Nuclear Membrane
Small Amount In The Cytoplasm And The Plasma Membrane
Exists Both As Soluble Cytoplasmic Protein And As Membrane Protein With Probably A Single Transmembrane Domain
Might Not Be Present In The Nucleus Of Cardiac Cells
Alternative Antibody Names Anti-Chloride Intracellular Channel Protein 1 antibody
Anti-Chloride Channel Abp antibody
Anti-Nuclear Chloride Ion Channel 27 antibody
Anti-Ncc27 antibody
Anti-Regulatory Nuclear Chloride Ion Channel Protein antibody
Anti-Hrncc antibody
Anti-CLIC1 antibody
Anti-G6 antibody
Anti-NCC27 antibody

Information sourced from Uniprot.org

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