Western blot analysis of lysates from NIH/3T3 cells using Cleaved PARP (Asp214) Rabbit mAb (STJ11107362) at 1:1000 dilutionย incubated overnight at 4C. NIH/3T3 cells were treated by staurosporine (1 Mu M) for 3 hour. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 90s.

Anti-Cleaved-PARP-Asp214 antibody (211-310 aa) [SRM5448] (STJ11107362)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11107362
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General Information

Short DescriptionRabbit monoclonal Cleaved-PARP-Asp214 (211-310 aa) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityMouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDSRM5448
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Immunogen Region211-310 aa
Immunogen SequenceDEVDGTDEVAKKKSKKGKDK DSSKLEKALKAQNELIWNIK DELKKACSTNDLKELLIFNQ QQVPSGESAILDRVADGMAF GALLPCKECSGQLVFKSDAY
SpecificityA synthetic peptide corresponding to a sequence within amino acids 211-310 of Mouse Cleaved PARP (Asp214) (NP_031441.2).

Additional Info

Background Enables NAD+ ADP-ribosyltransferase activity; chromatin binding activity; and protein ADP-ribosylase activity. Involved in negative regulation of telomere maintenance via telomere lengthening; positive regulation of single strand break repair; and voluntary musculoskeletal movement. Acts upstream of or within several processes, including DNA metabolic process; behavioral response to cocaine; and protein poly-ADP-ribosylation. Located in cytoplasm; nucleolus; and nucleoplasm. Part of protein-containing complex. Is expressed in several structures, including Peyer s patch; brain; gut; immune system; and retina. Human ortholog (s) of this gene implicated in several diseases, including arthritis; bacterial meningitis; neurodegenerative disease (multiple) ; stomach carcinoma; and type 2 diabetes mellitus. Orthologous to human PARP1 (poly (ADP-ribose) polymerase 1).

Information sourced from Uniprot.org

Citations

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