• Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Cleaved-ITGA3-Phe33, Heavy Chain antibody (32-33) (STJA0005871)

SKU:
STJA0005871

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IF/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cleaved-Integrin alpha-3-Phe33, Heavy Chain (32-33) is suitable for use in Western Blot, Immunofluorescence, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IF ICC 1:50-200
ELISA 1:2000-20000
IHC-p 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGA3
Gene ID: 3675
Uniprot ID: ITA3_HUMAN
Immunogen Region: 32-33
Specificity: This antibody detects endogenous levels of Human, Mouse, Rat ITGA3 (heavy chain, Cleaved-Phe33, protein was cleaved amino acid sequence between 32-33 )
Immunogen: Synthesized peptide derived from human ITGA3 (heavy chain, Cleaved-Phe33)
Tissue Specificity Isoform 1 is widely expressed. Isoform 2 is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform 1 is strongly expressed on vascular smooth muscle cells, isoform 2 is detected only on endothelial vein cells.
Post Translational Modifications Isoform 1, but not isoform 2, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation.
Function Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. (Microbial infection) Integrin ITGA3:ITGB1 may act as a receptor for R.delemar CotH7 in alveolar epithelial cells, which may be an early step in pulmonary mucormycosis disease progression.
Protein Name Integrin Alpha-3
Cd49 Antigen-Like Family Member C
Frp-2
Galactoprotein B3
Gapb3
Vla-3 Subunit Alpha
Cd Antigen Cd49c Cleaved Into - Integrin Alpha-3 Heavy Chain - Integrin Alpha-3 Light Chain
Database Links Reactome: R-HSA-210991
Reactome: R-HSA-216083
Reactome: R-HSA-3000157
Reactome: R-HSA-8874081
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Lipid-Anchor
Cell Projection
Invadopodium Membrane
Filopodium Membrane
Enriched Preferentially At Invadopodia
Cell Membrane Protrusions That Correspond To Sites Of Cell Invasion
In A Collagen-Dependent Manner
Alternative Antibody Names Anti-Integrin Alpha-3 antibody
Anti-Cd49 Antigen-Like Family Member C antibody
Anti-Frp-2 antibody
Anti-Galactoprotein B3 antibody
Anti-Gapb3 antibody
Anti-Vla-3 Subunit Alpha antibody
Anti-Cd Antigen Cd49c Cleaved Into - Integrin Alpha-3 Heavy Chain - Integrin Alpha-3 Light Chain antibody
Anti-ITGA3 antibody
Anti-MSK18 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance