| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Cleaved-Collagen alpha-1XI chain-Ala1563 (1563-1564) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000-2000ELISA 1:5000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
COL11A1 |
| Gene ID: |
1301 |
| Uniprot ID: |
COBA1_HUMAN |
| Immunogen Region: |
1563-1564 |
| Specificity: |
This antibody detects endogenous levels of Human, Mouse Collagen XI Alpha 1 (Cleaved-Ala1563, protein was cleaved amino acid sequence between 1563-1564 ) |
| Immunogen: |
Synthesized peptide derived from human Collagen XI Alpha 1 (Cleaved-Ala1563) |
| Tissue Specificity | Cartilage, placenta and some tumor or virally transformed cell lines. Isoforms using exon IIA or IIB are found in the cartilage while isoforms using only exon IIB are found in the tendon. |
| Post Translational Modifications | Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. N-glycosylated. |
| Function | May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils. |
| Protein Name | Collagen Alpha-1(Xi Chain |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1650814Reactome: R-HSA-2022090Reactome: R-HSA-3000171Reactome: R-HSA-8874081Reactome: R-HSA-8948216 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Names | Anti-Collagen Alpha-1(Xi Chain antibodyAnti-COL11A1 antibodyAnti-COLL6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and NG2/CSPG4 knockout (KO) HeLa cells, using [KO Validated] NG2/CSPG4 Rabbit polyclonal antibody (STJ29905) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/102949/392251/STJ29905_1__21734.1713162656.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NG2/CSPG4 knockout (KO) HeLa cells, using [KO Validated] NG2/CSPG4 Rabbit polyclonal antibody (STJ29905) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of lysates from wild type (WT) and NG2/CSPG4 Rabbit polyclonal antibody knockout (KO) HeLa cells, using [KO Validated] NG2/CSPG4 Rabbit polyclonal antibody (STJ11104656) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/177300/368955/STJ11104656_1__39824.1713134432.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NG2/CSPG4 Rabbit polyclonal antibody knockout (KO) HeLa cells, using [KO Validated] NG2/CSPG4 Rabbit polyclonal antibody (STJ11104656) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")