• Western blot analysis of Hela cell treated or untreated with 2 mM/ml camptothecin, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Cleaved-CASP10-Val220 antibody (STJ193237)

SKU:
STJ193237

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cleaved-Caspase-10-Val220 is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA-Pep 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CASP10
Gene ID: 843
Uniprot ID: CASPA_HUMAN
Specificity: This antibody detects endogenous levels of human Caspase 10 (p23/17, Cleaved-Val220)
Immunogen: Synthesized peptide derived from the human Caspase 10 (p23/17, Cleaved-Val220)
Post Translational Modifications Cleavage by granzyme B and autocatalytic activity generate the two active subunits.
Function Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP8 and CASP9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis. Isoform C is proteolytically inactive.
Protein Name Caspase-10
Casp-10
Apoptotic Protease Mch-4
Fas-Associated Death Domain Protein Interleukin-1b-Converting Enzyme 2
Flice2
Ice-Like Apoptotic Protease 4 Cleaved Into - Caspase-10 Subunit P23/17 - Caspase-10 Subunit P12
Database Links Reactome: R-HSA-6803207
Reactome: R-HSA-75157
Reactome: R-HSA-75158
Reactome: R-HSA-933543
Alternative Antibody Names Anti-Caspase-10 antibody
Anti-Casp-10 antibody
Anti-Apoptotic Protease Mch-4 antibody
Anti-Fas-Associated Death Domain Protein Interleukin-1b-Converting Enzyme 2 antibody
Anti-Flice2 antibody
Anti-Ice-Like Apoptotic Protease 4 Cleaved Into - Caspase-10 Subunit P23/17 - Caspase-10 Subunit P12 antibody
Anti-CASP10 antibody
Anti-MCH4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance