• Western blot analysis of lysates from mouse large intestine using CLDN7 rabbit polyclonal antibody (STJ23163) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:25s.
  • Immunofluorescence analysis of human colon using CLDN7 rabbit polyclonal antibody (STJ23163) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.
  • Immunofluorescence analysis of rat large intestine using CLDN7 rabbit polyclonal antibody (STJ23163) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.

Anti-CLDN7 antibody (29-211) (STJ23163)

SKU:
STJ23163

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLDN7 (29-211) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLDN7
Gene ID: 1366
Uniprot ID: CLD7_HUMAN
Immunogen Region: 29-211
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-211 of human CLDN7 (NP_001298.3).
Immunogen Sequence: QWQMSSYAGDNIITAQAMYK GLWMDCVTQSTGMMSCKMYD SVLALSAALQATRALMVVSL VLGFLAMFVATMGMKCTRCG GDDKVKKARIAMGGGIIFIV AGLAALVACSWYGHQIVTDF YNPLIPTNIKYEFGPAIFIG WAGSALVILGGALLSCSCPG NESKAGYRVPRSYPKSNSSK EYV
Tissue Specificity Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
Post Translational Modifications Phosphorylated.
Function Plays a major role in tight junction-specific obliteration of the intercellular space.
Protein Name Claudin-7
Cldn-7
Database Links Reactome: R-HSA-420029
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Cell Junction
Tight Junction
Co-Localizes With Epcam At The Basolateral Cell Membrane And Tight Junction
Alternative Antibody Names Anti-Claudin-7 antibody
Anti-Cldn-7 antibody
Anti-CLDN7 antibody
Anti-CEPTRL2 antibody
Anti-CPETRL2 antibody

Information sourced from Uniprot.org

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