• Western blot analysis of extracts of various cell lines, using CHRNA10 antibody (STJ23133) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-CHRNA10 antibody (25-240) (STJ23133)

SKU:
STJ23133

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHRNA10 (25-240) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHRNA10
Gene ID: 57053
Uniprot ID: ACH10_HUMAN
Immunogen Region: 25-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-240 of human CHRNA10 (NP_065135.2).
Immunogen Sequence: AEGRLALKLFRDLFANYTSA LRPVADTDQTLNVTLEVTLS QIIDMDERNQVLTLYLWIRQ EWTDAYLRWDPNAYGGLDAI RIPSSLVWRPDIVLYNKADA QPPGSASTNVVLRHDGAVRW DAPAITRSSCRVDVAAFPFD AQHCGLTFGSWTHGGHQLDV RPRGAAASLADFVENVEWRV LGMPARRRVLTYGCCSEPYP DVTFTLLLRRRAAAYV
Tissue Specificity Expressed in inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes.
Function Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma.
Protein Name Neuronal Acetylcholine Receptor Subunit Alpha-10
Nicotinic Acetylcholine Receptor Subunit Alpha-10
Nachr Alpha-10
Database Links Reactome: R-HSA-9667769
Cellular Localisation Postsynaptic Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Neuronal Acetylcholine Receptor Subunit Alpha-10 antibody
Anti-Nicotinic Acetylcholine Receptor Subunit Alpha-10 antibody
Anti-Nachr Alpha-10 antibody
Anti-CHRNA10 antibody
Anti-NACHRA10 antibody

Information sourced from Uniprot.org

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