• Anti-CHRM1 antibody (6-460) (STJ11101182)
  • Immunofluorescence analysis of NIH-3T3 cells using CHRM1 Rabbit polyclonal antibody (STJ11101182) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using CHRM1 Rabbit polyclonal antibody (STJ11101182) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of OVCAR-3 cells using CHRM1 Rabbit polyclonal antibody (STJ11101182) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CHRM1 Rabbit polyclonal antibody (STJ11101182) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHRM1 antibody (6-460) (STJ11101182)

SKU:
STJ11101182

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:5000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHRM1
Gene ID: 1128
Uniprot ID: ACM1_HUMAN
Immunogen Region: 6-460
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CHRM1 (NP_000729.2).
Immunogen Sequence: MNTSAPPAVSPNITVLAPGK GPWQVAFIGITTGLLSLATV TGNLLVLISFKVNTELKTVN NYFLLSLACADLIIGTFSMN LYTTYLLMGHWALGTLACDL
Function The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Protein Name Muscarinic Acetylcholine Receptor M1
Database Links Reactome: R-HSA-390648
Reactome: R-HSA-416476
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Postsynaptic Cell Membrane
Alternative Antibody Names Anti-Muscarinic Acetylcholine Receptor M1 antibody
Anti-CHRM1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance