• Western blot analysis of lysates from DU145 cells, using CHPT1 Rabbit polyclonal antibody (STJ118577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHPT1 antibody (1-100) (STJ118577)

SKU:
STJ118577

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHPT1 (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHPT1
Gene ID: 56994
Uniprot ID: CHPT1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CHPT1 (NP_064629.2).
Immunogen Sequence: MAAGAGAGSAPRWLRALSEP LSAAQLRRLEEHRYSAAGVS LLEPPLQLYWTWLLQWIPLW MAPNSITLLGLAVNVVTTLV LISYCPTATEEAPYWTYLLC
Tissue Specificity Highly expressed in testis, colon, small intestine, heart, prostate and spleen. Also detected in kidney, skeletal muscle, pancreas, leukocytes, ovary and thymus. Weakly expressed in the brain, placenta and lung. Overexpressed in cancerous breast epithelial cell lines.
Function Catalyzes the final step of de novo phosphatidylcholine (PC) synthesis, i.e. the transfer of choline phosphate from CDP-choline to the free hydroxyl of a diacylglycerol (DAG), producing a PC. It thereby plays a central role in the formation and maintenance of vesicular membranes.
Protein Name Cholinephosphotransferase 1
Hcpt1
Aapt1-Like Protein
Diacylglycerol Cholinephosphotransferase 1
Database Links Reactome: R-HSA-1483191
Cellular Localisation Golgi Apparatus Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Cholinephosphotransferase 1 antibody
Anti-Hcpt1 antibody
Anti-Aapt1-Like Protein antibody
Anti-Diacylglycerol Cholinephosphotransferase 1 antibody
Anti-CHPT1 antibody
Anti-CPT1 antibody
Anti-MSTP022 antibody

Information sourced from Uniprot.org

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