• Immunohistochemistry analysis of CHDH in paraffin-embedded human kidney using CHDH Rabbit polyclonal antibody (STJ118972) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using CHDH Rabbit polyclonal antibody (STJ118972) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) :JurkatExposure time: 1s.

Anti-CHDH antibody (245-594) (STJ118972)

SKU:
STJ118972

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHDH (245-594) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHDH
Gene ID: 55349
Uniprot ID: CHDH_HUMAN
Immunogen Region: 245-594
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 245-594 of human CHDH (NP_060867.2).
Immunogen Sequence: LHPALSRTNLKAEAETLVSR VLFEGTRAVGVEYVKNGQSH RAYASKEVILSGGAINSPQL LMLSGIGNADDLKKLGIPVV CHLPGVGQNLQDHLEIYIQQ ACTRPITLHSAQKPLRKVCI GLEWLWKFTGEGATAHLETG GFIRSQPGVPHPDIQFHFLP SQVIDHGRVPTQQEAYQVHV GPMRGTSVGWLKLRSANPQD HPVIQPNYLSTETDIEDFRL CVKLTREIFAQEALAPFRG
Protein Name Choline Dehydrogenase - Mitochondrial
Cdh
Chd
Database Links Reactome: R-HSA-6798163
Cellular Localisation Mitochondrion Inner Membrane
Alternative Antibody Names Anti-Choline Dehydrogenase - Mitochondrial antibody
Anti-Cdh antibody
Anti-Chd antibody
Anti-CHDH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance